Research Article
Immunoproteomic Analysis of Chinese Brucella Vaccine Strain M5 and New Immunogenic Proteins
Yanling Yang1,2, Jigang Yin3, Xinglong Wang2,3*, XiuranWang4, Fengxue Wang1,Yongjun Wen1, Hailong Qu2 and JiankeWang11The Eleventh Institute of the Academy of Military Medical Sciences of PLA, Changchun, 130062, China
2Institution of Special Wild Economic Animal and Plant Science, CAAS, Jilin 132109, China
3Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China
4School of Life Science, Jilin Agricultural University, 130118, China
- *Corresponding Author:
- Xinglong Wang
The Eleventh Institute of the Academy of Military Medical Sciences of PLA
Changchun, 130062, China
E-mail: [email protected]
Received date: April 05, 2012; Accepted date: June 14, 2012; Published date: June 18, 2012
Citation: Yang Y, Yin J, Wang X, Wang X, Wang F, et al. (2012) Immunoproteomic Analysis of Chinese Brucella Vaccine Strain M5 and New Immunogenic Proteins. J Data Mining Genomics Proteomics 3:117. doi:10.4172/2153-0602.1000117
Copyright: © 2012 Yang Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Brucellae are facultative intracellular gram-negative bacteria that cause human disease and significant worldwide economic loss due to infection of livestock. Available vaccines against Brucella spp. are live attenuated Brucella strains. In order to engineer a better vaccine to be used in animals and humans, our laboratory aims to develop an innocuous subunit vaccine. Particularly, we are interested in Brucella membrane proteins (MPs). In this study, an immunoproteomic approach was utilized to identify novel antigenic candidate proteins from Chinese Brucella vaccine strain M5 membrane proteins. The membrane proteins were separated by 2-DE and analyzed by western-blotting for their reactivity with the antiserum obtained from goats naturally infected by Brucella. Of a total of 9 immunogenic proteins identified, 7 were shown to be the novel antigens for Brucella spp. Some of the major protein components include outer-membrane protein OMP25, OMP31, and several new immunogenic proteins were identified, such as isovaleryl coenzyme A dehydrogenase, nitroglycerin reductase, succinyl-CoA synthetase subunit and S- adenosine -L- homocysteine hydrolase. Comparing these gene sequences revealed that 8 out of 9 immunoreactive protein genes were found in all 5 different Brucella strains. The elucidation of the immunome of Brucella vaccine strain M5 MPs identified a number of candidate proteins for developing vaccines against Brucella infection in livestock.
