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ISSN: 2329-9029

Journal of Plant Biochemistry & Physiology
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Research Article

Impact of Gold Nanoparticles on Physiological and Biochemical Characteristics of Brassica juncea

Gunjan B1*, Zaidi MGH2 and Sandeep A3
1Department of Natural Science, Kathmandu University, Dhulikhel, Nepal
2Department of Chemistry, G.B. Pant University of Agriculture & Technology, Pantnagar, India
3Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture & Technology, Pantnagar, India
Corresponding Author : Gunjan B
Department of Natural Science
Kathmandu University, Dhulikhel, Nepal
Tel: 00977-9818874898
E-mail: [email protected]
Received July 09, 2014; Accepted September 22, 2014; Published September 27, 2014
Citation: Gunjan B, Zaidi MGH, Sandeep A (2014) Impact of Gold Nanoparticles on Physiological and Biochemical Characteristics of Brassica juncea. J Plant Biochem Physiol 2:133. doi: 10.4172/2329-9029.1000133
Copyright: © 2014 Gunjan B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Agricultural crops are an essential base component of all ecosystems and it is important to assess the impact of nanoparticles on them and the risks involved. In this study we have investigated the impact of the different concentrations (ppm) of citrate stabilized gold nanoparticles (GNPs) ranging from 100 to 400 on physiological and biochemical parameters of the seedlings of Brassica juncea as a model oil crop system. GNPs were synthesized and characterized through Uv-visible spectra and transmission electron microscopy. The qualitative accumulation of GNPs into seedlings of Brassica juncea was ascertained through cyclic voltammetry and Laser Induced Breakdown Spectra (LIBS). Exposure of GNPs caused a decrease in overall growth of Brassica juncea was attributed to increase in free radical stress, supported by a general increase in biomarkers such as antioxidative enzymes, proline and hydrogen peroxide due to the formation of reactive oxygen species.

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