Improved Gastrodin Production of Biotransformation Conditions by Cultured Cells Armillaria luteo-virens Sacc and the Anti-inflammatory Activity In Vivo
- *Corresponding Author:
- Qi-He Chen
Department of Food Science and Nutrition
Zhejiang University, Yuhangtang Rd. 866
Hangzhou 310058, China
E-mail: [email protected]
Received date: February 19, 2016 Accepted date: March 29, 2016 Published date: March 31, 2016
Citation: Niu YW, Li HJ, Dong YC, Xu DQ, Chen QH (2016) Improved Gastrodin Production of Biotransformation Conditions by Cultured Cells Armillaria luteo-virens Sacc and the Anti-inflammatory Activity In Vivo. Med chem (Los Angeles) 6:211-217. doi:10.4172/2161-0444.1000348
Copyright: © 2016 Niu YW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Gastrodin (GAS), the main bioactive component of G. elata Blume, has important pharmaceutical and functional activities. The aim of this study is to produce GAS from p-2-hydroxybenzyl alcohol (HBA) through biotransformation. The conversion of exogenous HBA into GAS compound was conducted using cell suspension cultures of Armillaria luteo-virens Sacc. The bioconversion conditions were fully optimized with response surface methodology (RSM), turning out that the optimal transformation conditions composed of 3 mg/mL HBA, 6.5 g/30 mL inoculums level, 1.5% Tween 80, pH 4.5, and transformation temperature at 23°C. Under the optimized conditions, the conversion productivity of GAS reached the highest value (5.65 ± 0.45 mg/L). Verified experiments further validated that the optimized conditions were suitable for predicting the actual process of HBA transformation in the resting-cell system. The bioconversion kinetics model was as well simulated with Michaelis–Menten equation, which showing the suitability. The present study proposed the biotransformation pathway of HBA into GAS by resting cells transformation, indicating that the biotransformation process involved glucosylation reaction. Furthermore, Imprinting Control Region (ICR) mice in vivo demonstrated that the identified gastrodin possessed a significant anti-inflammatory activity. The fundamental data in the present work provides an efficient way to produce GAS through the whole-cells biocatalysis.