alexa Improved Immuno-Detection of a Low-Abundance Cyclophilin Allows the Confirmation of its Expression in a Protozoan Parasite | OMICS International | Abstract
ISSN: 2469-9756

Immunochemistry & Immunopathology
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Research Article

Improved Immuno-Detection of a Low-Abundance Cyclophilin Allows the Confirmation of its Expression in a Protozoan Parasite

Patricia L Bustos1*, Alina E Perrone1, Natalia A Milduberger1,2 and Jacqueline Bua1,2

1Instituto Nacional de Parasitología ‘‘Dr. Mario Fatala Chabén’’- A.N.L.I.S. Malbrán, Av. Paseo Colón 568, C1063ACS, Buenos Aires, Argentina

2CAECIHS, Universidad Abierta Interamericana, Av. Montes de Oca 745, 2° piso, C1270AAH, Buenos Aires, Argentina.

*Corresponding Author:
Patricia L. Bustos
Instituto Nacional de Parasitología ‘‘Dr. Mario Fatala Chabén’’
Av. Paseo Colón 568 C1063ACS
Ciudad Autónoma de Buenos Aires, Argentina
Tel: +541143314019
E-mail: [email protected]

Received date: September 16, 2015; Accepted date: October 01, 2015; Published date: October 06, 2015

Citation: Bustos PL, Perrone AE, Milduberger NA, Bua J (2015) Improved Immuno-Detection of a Low-Abundance Cyclophilin Allows the Confirmation of its Expression in a Protozoan Parasite. Immunochem Immunopathol: Open Access 1:103. doi: 10.4172/2469-9756.1000103

Copyright: © 2015 Bustos PL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Protein samples can be challenging to analyze due to the presence of high-abundance proteins masking lowabundance proteins of interest, such as biomarkers and novel physiological mediators. Cyclophilins are chaperones involved in the cis/trans isomerization of peptidyl-prolyl bonds in peptides or proteins and have been found in every organism sequenced to date. Although considerable progress has been made in the characterization of some cyclophilins expressed in diverse parasites invading humans, the main aspects of low-abundance members of this family remain unknown. In the present work, we present that the combined strategy of using more specific antibodies and increasing the presence of subcellular proteins in the sample, allowed us to confirm the expression of a 21.1 kDa cyclophilin for the first time in Trypanosoma cruzi.

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