Improvement of Lentigines by Oral Crocetin Administration and Examination of Its Mechanism of ActionHaifeng Zeng1, Lihao Gu1, Naofumi Umigai2, Reiko Maruya3, Takashi Koikeda4 and Kazuhisa Maeda1*
- *Corresponding Author:
- Kazuhisa Maeda
Tokyo University of Technology Graduate School
Hachioji City, Tokyo 192-0982, Japan
E-mail: [email protected]
Received Date: April 10, 2017; Accepted Date: April 13, 2017; Published Date: April 20, 2017
Citation: Zeng H, Gu L, Umigai N, Maruya R, Koikeda T, et al. (2017) Improvement of Lentigines by Oral Crocetin Administration and Examination of Its Mechanism of Action. J Nutr Food Sci 7:598. doi: 10.4172/2155-9600.1000598
Copyright: © 2017 Zeng H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
We investigated the effect of crocetin, a carotenoid present in the fruits of Gardenia jasminoides, on the improvement of lentigines in the skin and examined its mechanism of action. Subjects consumed an experimental meal consisting of a drink containing 7.5 mg of crocetin daily after dinner for 8 weeks. We examined the effects of oral ingestion of crocetin on the improvement of the size of lentigines on the skin. In an epidermis model in which crocetin was added at concentrations of 1 μg/mL and 2 μg/mL and cultured for 14 days, melanin was significantly suppressed compared with the control. Investigation of the mechanism of action via a cultured human epidermis model and cultured pigment cells revealed that crocetin decreased melanocortin receptor subtype 1 of melanocytes at the messenger ribonucleic acid level and inhibited the stimulatory action of melanocytes via melanocyte-stimulating hormone. The results of the study concluded that crocetin inhibits melanin synthesis by controlling the synthesis of tyrosinase and tyrosinase-related protein-1 via the suppression of melanocortin receptor subtype 1 expression.