Improving Cardiac Action Potential Measurements: 2D and 3D Cell Culture
Neil J Daily, Yue Yin, Pinar Kemanli, Brian Ip, and Tetsuro Wakatsuki*
InvivoSciences, Inc., Madison, WI 53719, USA
- *Corresponding Author:
- Tetsuro Wakatsuki
Chief Scientific Officer
InvivoSciences, Inc., Madison
WI 53719, USA
E-mail: [email protected]
Received Date: September 29, 2015; Accepted Date: October 05, 2015; Published Date: October 16, 2015
Citation: Daily NJ, Yin Y, Kemanli P, Brian Ip, Wakatsuki T (2015) Improving Cardiac Action Potential Measurements: 2D and 3D Cell Culture. J Bioengineer & Biomedical Sci 5:168. doi:10.4172/2155-9538.1000168
Copyright: © 2015 Daily NJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Progress in the development of assays for measuring cardiac action potential is crucial for the discovery of drugs for treating cardiac disease and assessing cardiotoxicity. Recently, high-throughput methods for assessing action potential using induced pluripotent stem cell (iPSC) derived cardiomyocytes in both two-dimensional monolayer cultures and three-dimensional tissues have been developed. We describe an improved method for assessing cardiac action potential using an ultra-fast cost-effective plate reader with commercially available dyes. Our methods improve dramatically the detection of the fluorescence signal from these dyes and make way for the development of more high-throughput methods for cardiac drug discovery and cardiotoxicity.