alexa In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide Trxaq-AMP Obtained from Amaranthus quitensis
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide Trxaq-AMP Obtained from Amaranthus quitensis

Diego Alem1, Paola Díaz-Dellavalle2, Carolina Leoni3, Salvatore G De-Simone4,5, Agustín Correa6, Pablo Oppezzo6 and Marco Dalla Rizza1*

1Laboratorio de Proteínas, Unidad de Biotecnología, Estación Experimental Wilson Ferreira Aldunate, Instituto Nacional de Investigación Agropecuaria (INIA Las Brujas), Canelones, Uruguay

2Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay

3Sección Protección Vegetal, Programa Nacional de Investigación de Producción y Sustentabilidad Ambiental, Estación Experimental Wilson Ferreira Aldunate, Instituto Nacional de Investigación Agropecuaria (INIA Las Brujas), Canelones, Uruguay

4Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Diseases (INCT-IDN), FIOCRUZ, Rio de Janeiro, RJ, Brazil

5Biochemistryof Proteins and Peptides Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, RJ, Brazil.

6Unidad de Proteínas Recombinantes – Institut Pasteur de Montevideo, Uruguay

*Corresponding Author:
Marco Dalla Rizza
Laboratorio de Proteínas, Unidad de Biotecnología
Estación Experimental Wilson Ferreira Aldunate
Instituto Nacional de Investigación Agropecuaria (INIA Las Brujas)
Canelones, Uruguay
Tel: +598 3677641
Fax: 598 3677609
E-mail: [email protected]

Received date: June 02, 2014; Accepted date: June 16, 2014; Published date: June 24, 2014

Citation: Alem D, Díaz-Dellavalle P, Leoni C, De-Simone SG, Correa A, et al. (2014) In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP Obtained from Amaranthus quitensis. J Microb Biochem Technol 6:268-273. doi: 10.4172/1948-5948.1000155

Copyright: © 2014 Alem D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq-AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present an AMP with gathering no hemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, pH-conditions and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easily to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially those in relation related to molecule bioavailability.

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