Journal of Proteomics & Bioinformatics
Open Access

Abstract

In Silico and In Vivo Study of Lens Regeneration Under theInfluence of Retinoid

Amit Nagal, O.P. Jangir, P S Solanki, Vijay Singh and M Krishnamohan

The present study supports previous finding that vitamin A can induce and accelerate lens regeneration in pigmented epithelial cells (PECs) of dorsal iris not only in amphibians but also in young and adult swiss albino mice, guinea pig, rabbit and pigs. In lens regeneration, several workers have shown that vitamin A possesses the mitogenic activity which causes functional impairment of retinoid receptors and thereby inhibits the lens regeneration. The purpose of present study is to know how retinoids and their derivatives interact with Retinoid X receptor (Rxr alpha) and thus helping in lens regeneration. The docking studies of human and mice Rxr alpha were performed against vitamin A and 9-cis retinoic acid (vitamin A1) using autodock and the results were analyzed using Discovery Studio from Accelrys. The results show that there is a significant similarity in interaction energy of Rxr alpha of mice and human. The highest rank docked energy of Rxr alpha mice with vitamin A was -11.65 kcal, which was much closed to -11.83 kcal of 9-cis retinoic acid. Similarly, in human Rxr alpha receptor, the highest docked energy showed the significant similarity with vitamin A (-12.19 kcal) and 9- cis retinoic acid (-12.14 kcal). This study suggests that vitamin A shows effect on proliferation and differentiation similar to the 9-cis retinoic acid and also proves that vitamin A acts on Retinoid X alpha receptors and enhance lens regeneration in mammals.