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<em>In Silico</em> Prediction of the Tertiary Structure of <em>M. leprae</em> Hsp65 Protein Shows an Unusual Structure in Carboxy-terminal Region | OMICS International | Abstract
ISSN: 0974-7230

Journal of Computer Science & Systems Biology
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Research Article

In Silico Prediction of the Tertiary Structure of M. leprae Hsp65 Protein Shows an Unusual Structure in Carboxy-terminal Region

Rossetti RAM1,2, Lorenzi JCC1, Giuliatti, S1, Silva CL1, Coelho-Castelo AAM1*

 1Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.

2Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, University of São Paulo , Brazil

*Corresponding Author:
Dr. Coelho-Castelo AAM
Departamento de Bioquimica e Imunologia, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP,
Phone : 55-16-36024532 / 36024538,
Email  : [email protected]

Received Date: December 14, 2008; Accepted Date: December 24, 2008; Published Date: December 26, 2008

Citation: Rossetti RAM, Lorenzi JCC, Giuliatti S, Silva CL, Coelho C AAM (2008) In Silico Prediction of the Tertiary Structure of M. leprae Hsp65 Protein Shows an Unusual Structure in Carboxy-terminal Region. J Comput Sci Syst Biol 1:126-131. doi: 10.4172/jcsb.1000012

Copyright: © 2008 Rossetti RAM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

DNA vaccines have been used with great success in experimental and some clinical therapy. However, the mechanisms of activation of the immune system by these vaccines are not utterly understood yet. Hsp65 is aMycobacterium leprae chaperone whose gene has been efficiently used as experimental DNA vaccine against tuberculosis and clinical trial against tumor. Since little is know about the three-dimensional (3D) structure of hsp65 and modeling of 3D protein structure can increase the information to improve the knowledge about the mechanism action as well as the design of new DNA vaccine formulation, here we used the bioinformatics to get the design in silico of hsp65 (heat shock protein) molecule. The determination of hsp65 3D structure was obtained by homology using the software Modeller (Eswar et al., 2001). It was used two proteins as models: 1SJP, a 60- kDa chaperonin from Mycobacterium tuberculosis in the PDB, and the 1WE3, the crystal structure of the chaperonin complex Cpn60/Cpn10/(ADP)7 from Thermus thermophilus). Our results showed an interesting structure in Hsp65 that could be important in development or modulation of immune response.

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