In Vitro and In Vivo Biotinylation of Endothelial Cell Surface Proteins in the Pursuit of Targets for Vascular Therapies for Brain AVMsMargaret Simonian1*, Mark P Molloy2 and Marcus A Stoodley1
- *Corresponding Author:
- Margaret Simonian
Australian School of Advanced Medicine
Sydney, 2109, Australia
E-mail: [email protected]
Received date: April 17, 2012; Accepted date: April 28, 2012; Published date: April 30, 2012
Citation: Simonian M, Molloy MP, Stoodley MA (2012) In Vitro and In Vivo Biotinylation of Endothelial Cell Surface Proteins in the Pursuit of Targets for Vascular Therapies for Brain AVMs. Metabolomics S1:007. doi: 10.4172/2153-0769.S1-007
Copyright: © 2012 Simonian M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Identification of membrane proteins that are expressed on the arteriovenous malformation (AVM) endothelium post radiosurgery is of fundamental importance in developing a new treatment of brain AVMs. We successfully optimized and then employed in vitro and in vivo biotinylation methodology of murine cerebral endothelial cell cultures (bEnd.3) and the rat model of AVM to label membrane proteins. Those membrane proteins were then captured on streptavidin resin and identified using proteomics analysis. This is the first time that proteomics has been employed in the study of AVMs.