In Vitro Anti-Acetylcholinesterase Activity of Dichloromethane Leaf Extracts of Carphalea glaucescens in Chilo partellus Larvae
- *Corresponding Author:
- Anne W. Njoroge
Department of Biochemistry and Biotechnology
P.O. Box 43844-00100, Nairobi
E-mail: [email protected]
Received Date: February 18, 2016; Accepted Date: April 01, 2016; Published Date: April 04, 2016
Citation: Anne NW, Piero NM, Umar A, Felix M, Maina GS, et al. (2016) In Vitro Anti-Acetylcholinesterase Activity of Dichloromethane Leaf Extracts of Carphalea glaucescens in Chilo partellus Larvae. Biochem Anal Biochem 5:264. doi:10.4172/2161-1009.1000264
Copyright: © 2016 Anne AW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Acetlycholinesterase (AChE) hydrolyses the neurotransmitter acetylcholine resulting in the termination of nerve impulse at the synapse. Anti-acetylcholinesterase activities stop the passage of the nerve impulse at the synapse resulting in continuous stimulation which can lead to death. The manufacturers of many pesticides target the AChE because it interferes with the passage of the nerve impulse. In vitro study revealed that DCM leaf extract of Carphalea glaucescens has anti-acetylcholinesterase activity against crude acetlycholinesterase (AChE) enzyme extracted from Chilo partellus and an IC50 of 12.02 mg/ml was calculated. After qualitative phytochemical screening was carried out the phytochemicals which were present were tannins, phenols, flavonoids, steroids, terpenoids and alkaloids.