alexa In Vitro Antifungal Activity of Crude Hydro-Alcoholic Extract of Petiveria alliacea L on Clinical Candida Isolates
ISSN: 2327-5073

Clinical Microbiology: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

In Vitro Antifungal Activity of Crude Hydro-Alcoholic Extract of Petiveria alliacea L on Clinical Candida Isolates

María Teresa Illnait-Zaragozí1, Rosario Esperanza Velar Martínez1, José Illnait Ferrer2, Carlos Manuel Fernández Andreu1, Gerardo Félix Martínez Machín1,Mayda Rosa Perurena Lancha1, Ernesto Xavier Monroy-Vaca1 and Jacques F Meis3*

1Mycology Laboratory; Tropical Medicine Institute “Pedro Kourí” (IPK), Havana, Cuba

2Natural Products Laboratory; National Centre for Scientific Research (CNIC), Havana, Cuba

3Regional Public Health Laboratory Service, Netherlands

*Corresponding Author:
Jacques F. Meis
Regional Public Health Laboratory Service
Department of Medical Microbiology and Infectious Diseases
Canisius Wilhelmina Hospital (CWZ)
Weg door Jonkerbos 100 6532 SZ Nijmegen, Netherlands
Tel: 31 243657514
Fax: 31 243657516
E-mail: [email protected]

Received Date: May 05, 2014; Accepted Date: July 07, 2014; Published Date: July 20, 2014

Citation: Illnait-Zaragozí MT, Martínez REV, Ferrer JI, Andreu CMF, Machín GFM, et al. (2014) In Vitro Antifungal Activity of Crude Hydro- Alcoholic Extract of Petiveria Alliacea L on Clinical Candida Isolates. Clin Microbial 3:159. doi: 10.4172/2327-5073.1000159

Copyright: © 2014 Meis JF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: The global burden of infections due to Candida and the emerging resistance to antifungals has led to the search for new therapeutic alternatives. The aim of the present work it was to evaluate the in vitro antifungal activity of crude hydro-alcoholic extract of Petiveria alliacea L (HAEPAL) versus fluconazole against Candida isolates.
Methods: In vitro antifungal activity was evaluated by broth microdilution method in front of 125 Candida isolates (60 C. albicans). Inoculum of 1.5×106 CFU/mL in sterile saline solution were incubated with five dilutions of the extract (128, 64, 32, 16 and 8 μg/mL). The minimum inhibitory concentration (MIC) was defined as the lowest concentration of HAEPAL showing ≤ 50% growth compared with the extract-free growth control estimated by counting the CFU/mL. Fluconazole susceptibility was assessed by ATBTM Fungus 3 and E-test according to manufacturer’s instructions.
Results: HAEPAL showed higher antifungal activity compared to fluconazole. Only four isolates (one C. albicans, one C. glabrata and two C. krusei) exhibited high MICs (≥ 64 μg/mL) compared to 34 (19 C. albicans and 15 Candida non-albicans) which showed resistance to fluconazole.
Conclusion: These results show the antifungal potentiality of HAEPAL, which could become a potential alternative for Candida treatment.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]line.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords