In Vitro Antifungal Activity of Crude Hydro-Alcoholic Extract of Petiveria alliacea L on Clinical Candida IsolatesMaría Teresa Illnait-Zaragozí1, Rosario Esperanza Velar Martínez1, José Illnait Ferrer2, Carlos Manuel Fernández Andreu1, Gerardo Félix Martínez Machín1,Mayda Rosa Perurena Lancha1, Ernesto Xavier Monroy-Vaca1 and Jacques F Meis3*
- *Corresponding Author:
- Jacques F. Meis
Regional Public Health Laboratory Service
Department of Medical Microbiology and Infectious Diseases
Canisius Wilhelmina Hospital (CWZ)
Weg door Jonkerbos 100 6532 SZ Nijmegen, Netherlands
Tel: 31 243657514
Fax: 31 243657516
E-mail: [email protected]
Received Date: May 05, 2014; Accepted Date: July 07, 2014; Published Date: July 20, 2014
Citation: Illnait-Zaragozí MT, Martínez REV, Ferrer JI, Andreu CMF, Machín GFM, et al. (2014) In Vitro Antifungal Activity of Crude Hydro- Alcoholic Extract of Petiveria Alliacea L on Clinical Candida Isolates. Clin Microbial 3:159. doi: 10.4172/2327-5073.1000159
Copyright: © 2014 Meis JF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: The global burden of infections due to Candida and the emerging resistance to antifungals has led to the search for new therapeutic alternatives. The aim of the present work it was to evaluate the in vitro antifungal activity of crude hydro-alcoholic extract of Petiveria alliacea L (HAEPAL) versus fluconazole against Candida isolates.
Methods: In vitro antifungal activity was evaluated by broth microdilution method in front of 125 Candida isolates (60 C. albicans). Inoculum of 1.5×106 CFU/mL in sterile saline solution were incubated with five dilutions of the extract (128, 64, 32, 16 and 8 μg/mL). The minimum inhibitory concentration (MIC) was defined as the lowest concentration of HAEPAL showing ≤ 50% growth compared with the extract-free growth control estimated by counting the CFU/mL. Fluconazole susceptibility was assessed by ATBTM Fungus 3 and E-test according to manufacturer’s instructions.
Results: HAEPAL showed higher antifungal activity compared to fluconazole. Only four isolates (one C. albicans, one C. glabrata and two C. krusei) exhibited high MICs (≥ 64 μg/mL) compared to 34 (19 C. albicans and 15 Candida non-albicans) which showed resistance to fluconazole.
Conclusion: These results show the antifungal potentiality of HAEPAL, which could become a potential alternative for Candida treatment.