alexa In vitro Antifungal activity of leaf extracts from Aloe secundiflora, Bulbine frutescens, Vernonia lasiopus and Tagetes minuta against Candida albicans
ISSN: 2167-0412

Medicinal & Aromatic Plants
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Research Article

In vitro Antifungal activity of leaf extracts from Aloe secundiflora, Bulbine frutescens, Vernonia lasiopus and Tagetes minuta against Candida albicans

Rachuonyo HO1*, Gatheri GW2, Ogola PE3, Arika WM3, Wambani JR4, Kebira AN1 and Kamau DN1
1Department of Microbiology, Kenyatta University, P.O Box 43 844-00100, Nairobi, Kenya
2Department of Plant sciences, Kenyatta University, P.O Box 43 844-00100, Nairobi, Kenya
3Department of Biochemistry and Biotechnology, Kenyatta University, P.O Box 43 844-00100, Nairobi, Kenya
4Department of Medical Laboratory Sciences, Kenyatta University, P.O Box 43 844-00100, Nairobi, Kenya
Corresponding Author : Rachuonyo HO
Department of Microbiology Kenyatta University
P.O Box 43 844-00100 Nairobi, Kenya
Tel: +254715407214
E-mail: [email protected]
Received January 08, 2016; Accepted January 20, 2016; Published January 23, 2016
Citation: Rachuonyo HO, Gatheri GW, Ogola PE, Arika WM, Wambani JR, et al. (2016) In vitro Antifungal activity of leaf extracts from Aloe secundiflora, Bulbine frutescens, Vernonia lasiopus and Tagetes minuta against Candida albicans. Med Aromat Plants 5:229. doi:10.4172/2167-0412.1000229
Copyright: © 2016 Rachuonyo HO, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Crude leaf extracts from medicinal plants were screened for in vitro antifungal properties against clinical isolate of Candida albicans. The antifungal test was carried out using the Kirby Bauer method. All the extracts from the plants tested showed antifungal activity against clinical isolate of Candida albicans with the extract from Vernonia lasiopus being more active as compared to the others. The minimum inhibitory concentration ranged from 4.0 to 8.9 mg/ml. The standard antifungal used as positive control was fluconazole (15 μg/ml) whereas dimethyl sulphoxide and distilled water were used as negative control. Preliminary screening for the presence of phytochemicals was also done to determine if there was presence of any, flavonoids, saponins, tannins and alkaloids.

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