alexa In Vitro Cholesterol Assimilation and Functional Enzymatic Activities of Putative Probiotic Lactobacillus sp. Ä°solated from Fermented Foods/ Beverages of North West India
ISSN: 2155-9600

Journal of Nutrition & Food Sciences
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

In Vitro Cholesterol Assimilation and Functional Enzymatic Activities of Putative Probiotic Lactobacillus sp. Ä°solated from Fermented Foods/ Beverages of North West India

Anila K, Kunzes A and Bhalla TC*

Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, Himachal Pradesh, India

*Corresponding Author:
Bhalla TC
Coordinator, Department of Biotechnology
Himachal Pradesh University, Summerhill
Shimla-171005, India
Tel: 911772832154
Fax: 911772832154, 911772832153
E-mail: [email protected]

Received Date: January 20, 2016;Accepted Date: Febuary 01, 2016; Published Date: Febuary 08, 2016

Citation: Anila K, Kunzes A, Bhalla TC (2016) In Vitro Cholesterol Assimilation and Functional Enzymatic Activities of Putative Probiotic Lactobacillus Sp. Isolated from Fermented Foods/Beverages of North West India. J Nutr Food Sci 6:467. doi: 10.4172/2155-9600.1000467

Copyright: © 2016 Anila K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



In the present investigation 5 putative probiotic lactobacilli strains isolated from ethnic fermented foods from tribal regions in North West India were explored for their capabilities to assimilate cholesterol in the presence of various bile salts. In addition, β-Galactosidase, β-Glucosidase, protease, amylase, phytase activity of lactobacilli strains were also evaluated. All the lactobacilli strains were able assimilate cholesterol and it was found to be significantly higher in the presence of bile salts. The highest cholesterol removal (47.70 μg/ml) was observed by L. casei PLA12 and L. casei PLA5 in the presence of cholic acid. Cholesterol removal by resting and dead cells was also investigated and it was found to be 2.08 and 4.74 μg/ml respectively. L. casei PLA5 produced highest β-glucosidase (14.97 ± 0.2 U/mg dcw); proteolytic (26 ± 0.5 U/mg) and phytase (23 ± 0.5 mm) activity and L. casei PLA12 was reported to produce highest β-galactosidase (0.80 ± 0.01 U/mg dcw) and amylase (13.06 ± 0.25 U/mg) activity. Moreover, none of the strains showed haemolytic, urease and bile salt hydrolase activity. Screening for enzymatic activities among lactic acid bacteria is important for the preparation and improvement of biological activity of food product and in the selection of microorganisms used in the growing industry of functional foods and beverages. This paper is the first report on functional characterization on lactic acid bacteria from some lesser-known ethnic fermented products of the North West Himalayas.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version