alexa In vitro Culture and Histological Characterization of E
ISSN: 2155-9554

Journal of Clinical & Experimental Dermatology Research
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Research Article

In vitro Culture and Histological Characterization of Extracted Human Hair Follicles

Rattapon Thuangtong1, Pitchaya Maneeprasopchoke1*, Chatchawan Srisawat2, Chinnavuth Vatanashevanopakorn2, Suchanan Hanamornroongruang3, Sarawut Junnu2 and Saroj Suvanasuthi1

1Department of Dermatology, Faculty of Medicine Siriraj hospital, Mahidol University, Thailand

2Department of Biochemistry, Faculty of Medicine Siriraj hospital, Mahidol University, Thailand

3Department of Pathology, Faculty of Medicine Siriraj hospital, Mahidol University, Thailand

*Corresponding Author:
Pitchaya Maneeprasopchoke
Department of Dermatology, Siriraj Hospital
2 Prannok Rd, Bangkoknoi, Bangkok, Thailand
Tel: +662-419-4333
Email: [email protected]

Received date: July 25, 2013; Accepted date: August 30, 2013; Published date: September 10, 2013

Citation: Thuangtong R, Maneeprasopchoke P, Srisawat C, Vatanashevanopakorn C, Hanamornroongruang S, et al. (2013) In vitro Culture and Histological Characterization of Extracted Human Hair Follicles. J Clin Exp Dermatol Res 4:182. doi: 10.4172/2155-9554.1000182

Copyright: © 2013 Thuangtong R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: In vitro-cultured hair, follicles can serve as an excellent model system for exploring the hair follicle biology as well as drug delivery targets. In this study, we aimed to investigate how to maintain whole human hair follicles in vitro and histologically characterize the hair follicles thereof.

Methods: Donated hair follicle specimens from hair transplant operation were cultured in vitro using a medium consisting of Williams` medium E, L-glutamine, insulin, hydrocortisone, penicillin, streptomycin, and amphotericin B. The growth of hair follicles was examined daily using a digital microscope. Their histological features were studied at day 0, 7, and 14 to characterize the changes of hair follicles maintained in culture.

Results: Cultured hair follicles continued to grow at a rate of about 200 μm/day during the first four days. Then the growth rate declined and stopped after an average (range) of 8.4 (7-11) days in culture. Histological study showed an upward regression of the hair follicles with scattered apoptosis of outer root sheath cells, resembling the follicles in a catagen stage.

Conclusion: Human hair follicles can be cultured in vitro for at least 7 days before progressing to catagen.

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