alexa <em>In vitro</em> Culture and Histological Characterization of Extracted Human Hair Follicles | OMICS International | Abstract
ISSN: 2155-9554

Journal of Clinical & Experimental Dermatology Research
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

In vitro Culture and Histological Characterization of Extracted Human Hair Follicles

Rattapon Thuangtong1, Pitchaya Maneeprasopchoke1*, Chatchawan Srisawat2, Chinnavuth Vatanashevanopakorn2, Suchanan Hanamornroongruang3, Sarawut Junnu2 and Saroj Suvanasuthi1

1Department of Dermatology, Faculty of Medicine Siriraj hospital, Mahidol University, Thailand

2Department of Biochemistry, Faculty of Medicine Siriraj hospital, Mahidol University, Thailand

3Department of Pathology, Faculty of Medicine Siriraj hospital, Mahidol University, Thailand

*Corresponding Author:
Pitchaya Maneeprasopchoke
Department of Dermatology, Siriraj Hospital
2 Prannok Rd, Bangkoknoi, Bangkok, Thailand
Tel: +662-419-4333
Email: [email protected]

Received date: July 25, 2013; Accepted date: August 30, 2013; Published date: September 10, 2013

Citation: Thuangtong R, Maneeprasopchoke P, Srisawat C, Vatanashevanopakorn C, Hanamornroongruang S, et al. (2013) In vitro Culture and Histological Characterization of Extracted Human Hair Follicles. J Clin Exp Dermatol Res 4:182. doi: 10.4172/2155-9554.1000182

Copyright: © 2013 Thuangtong R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Background: In vitro-cultured hair, follicles can serve as an excellent model system for exploring the hair follicle biology as well as drug delivery targets. In this study, we aimed to investigate how to maintain whole human hair follicles in vitro and histologically characterize the hair follicles thereof.

Methods: Donated hair follicle specimens from hair transplant operation were cultured in vitro using a medium consisting of Williams` medium E, L-glutamine, insulin, hydrocortisone, penicillin, streptomycin, and amphotericin B. The growth of hair follicles was examined daily using a digital microscope. Their histological features were studied at day 0, 7, and 14 to characterize the changes of hair follicles maintained in culture.

Results: Cultured hair follicles continued to grow at a rate of about 200 μm/day during the first four days. Then the growth rate declined and stopped after an average (range) of 8.4 (7-11) days in culture. Histological study showed an upward regression of the hair follicles with scattered apoptosis of outer root sheath cells, resembling the follicles in a catagen stage.

Conclusion: Human hair follicles can be cultured in vitro for at least 7 days before progressing to catagen.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7