In Vitro Cytotoxicity of Native and Rec-Pediocin CP2 Against Cancer Cell Lines: A Comparative StudyBalvir Kumar1*, Praveen P Balgir1, Baljinder Kaur1, Bharti Mittu1 and Ashish Chauhan2
- *Corresponding Author:
- Balvir Kumar
Department of Biotechnology
Punjabi University, Patiala, Punjab-147002, India
E-mail: [email protected]
Received date: July 27, 2012; Accepted date: November 02, 2012; Published date: November 05, 2012
Citation: Kumar B, Balgir PP, Kaur B, Mittu B, Chauhan A (2012) In Vitro Cytotoxicity of Native and Rec-Pediocin CP2 Against Cancer Cell Lines: A Comparative Study. Pharmaceut Anal Acta 3:183. doi: 10.4172/2153-2435.1000183
Copyright: © 2012 Kumar B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Pediocin CP2 is a natural antimicrobial peptide produced by Pediococcus acidilactici MTCC 5101. Its recombinant version was created by employing computational protein engineering approach and was expressed as a synthetic fusion protein in recombinant E. coli BL21(DE3)-pedA. Both native and rec-pediocin were comparatively evaluated for their cytotoxicity against HepG2 (a hepatocarcinoma cell line), HeLa (a cervical adenocarcinoma), MCF7 (a mammary gland adenocarcinoma) and Sp2/0-Ag14 (a spleen lymphoblast) cell lines. Inhibition of cell proliferation was quantitated by MTT assay and induction of apoptosis was studied by genomic DNA fragmentation assay. Results indicated a significantly higher cytotoxicity of rec-pediocin and damage of chromosomal DNA in bacteriocin tested cell lines.