alexa In Vitro Evaluation of Prebiotics on Adherence of Lactobacilli
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

In Vitro Evaluation of Prebiotics on Adherence of Lactobacilli

Krausova G*, Hyrslova I, Jakubec M and Hynstova I

Dairy Research Institute, Ke Dvoru 12a, 160 00 Prague, Czech Republic

*Corresponding Author:
Krausova G
Dairy Research Institute
Ke Dvoru 12a, 160 00 Prague
Czech Republic
Tel: +420 296 792 111
E-mail: [email protected]

Received Date: December 03, 2015; Accepted Date: December 18, 2015; Published Date: December 28, 2015

Citation: Krausova G, Hyrslova I, Jakubec M, Hynstova I (2016) In Vitro Evaluation of Prebiotics on Adherence of Lactobacilli. J Microb Biochem Technol 8: 006-008. doi:10.4172/1948-5948.1000253

Copyright: © 2015 Krausova G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



The ability to adhere to intestinal epithelia is a crucial probiotic property since the retention of bacteria in the large intestine is important for probiotic function. Previously, we have shown that the presence of prebiotics decreases the adherence in several prebiotic- probiotic combinations. The objective of this study was to test the effect of three commercially available prebiotics: Orafti GR, Orafti P95, and Orafti Synergy on the adherence of five Lactobacillus strains (Lactobacillus delbrueckii subsp. bulgaricus CCDM 66, Lactobacillus casei subsp. paracasei PE1TB-P, Lactobacillus fermentum RL25, Lactobacillus animalis CCDM 382, and Lactobacillus gasseri PHM-7E1). Adherence was tested using microtiter plates and was evaluated as the percentage of fluorescently labelled bacteria present in the wells after three washes. Adherence was evaluated in both uncoated and mucin-coated polystyrene plates. In accordance with our previous results, the majority of probiotic and prebiotic interactions resulted in at least a 10-fold decrease in adherence. Only one strain, L. gasseri PHM-7E1, exhibited increased adherence to mucin (from 5% to 8% and from 5% to 9%) after the addition of the prebiotics Orafti P95 and GR, respectively. These two combinations appear suitable for further synbiotic testing.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materia[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version