alexa In Vivo Biocompatibility Testing of a Collagen Cell Carrier Seeded with Human Urothelial Cells in Rats | OMICS International | Abstract
ISSN: 2157-7013

Journal of Cell Science & Therapy
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Research Article

In Vivo Biocompatibility Testing of a Collagen Cell Carrier Seeded with Human Urothelial Cells in Rats

Lisa Daum1, Sabine Maurer1, Martin Vaegler1and Karl-Dietrich Sievert1,2,3*

1Laboratory of Tissue Engineering, Department of Urology, Eberhard Karls University, Tübingen, Germany

2Department of Urology, Eberhard Karls University, Tübingen, Germany

3Department of Urology, University of Lübeck, Germany

*Corresponding Author:
Karl-Dietrich Sievert
MD PhD, Universitat zu Lubeck Lubeck
Germany
Tel:
+ 494515002271
E-mail:
[email protected]

Received Date: March 24, 2015 <strong>Accepted Date:</strong>: July 15, 2015 Published Date: July 18, 2015

Citation:Daum L, Maurer S, Vaegler M, Sievert K (2015) In Vivo Biocompatibility Testing of a Collagen Cell Carrier Seeded with Human Urothelial Cells in Rats. J Cell Sci Ther 6:215. doi:10.4172/2157-7013.1000215

Copyright: ©2015 Daum L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Tissue-engineered, matrix-stabilized autologous urothelium is a new option for urethral reconstruction, particularly for patients for whom other autologous grafts are not available. In vitro engineering of urothelial tissue requires biomaterials as cell carriers that increase the stability of cell-based implants. The aim of this study was to investigate a new highly standardized, industrially manufactured bovine collagen type I-based cell carrier (CCC) for its suitability as a carrier matrix for human urothelial cells (HUC). As an in vivo biocompatibility test the behaviour and degradation of these implants was to be proven in a nude rat model. Expanded HUC from tissue biopsies were phenotypically analysed by immuno¬cyto¬chemistry and seeded onto CCC. For in vivo application, CCC was seeded with PKH26-labelled HUC in high density and constructs were implanted onto the rectus abdominis muscle of nude rats. Integration, cell survival, and degradation of the urothelium-matrix-implants were studied after 1, 2, and 4 weeks. Immunohistological characterization of multilayered urothelium-matrix-constructs was performed for AE1/AE3 and p63 (epithelial phenotype), CK20 (differentiation), and E-Cadherin and ZO-1 (junction formation). Immunocytochemical staining showed urothelial character of the isolated cells and the absence of fibroblasts and muscle cells. In twelve nude rats, urothelium-matrix-implants integrated well into the host tissue where no inflammation was observed. Immuno¬fluorescence analysis confirmed epithelial phenotype, adherence and tight junction formation homogeneous multilayer formation of stratified HUC cultures as well as urothelial phenotype and differentiation in vitro the CCC-matrix additionally revealed promising biocompatibility investigated in a nude rat model. Thus, this study demonstrates excellent suitability of CCC as a matrix for urothelial cells and recommends its use in a large animal model with regard to clinical application.

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