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ISSN: 2329-6798

Modern Chemistry & Applications
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Research Article

Incidence of Listeria monocytogenes in Meat Product Samples by Real-Time PCR

Miroslava Kačániová1*, Maciej Kluz2, Jana Petrová1, Martin Mellen3, Simona Kunová1, Peter HašÄík1and Ľubomír Lopašovský1
1Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic
2Department of Biotechnology and Microbiology, University of Rzeszow, Rzeszow, Poland
3Hydina Slovakia, s.r.o., Nová Ľubovňa 505, Nová Ľubovňa 065 11, Slovakia
Corresponding Author : Miroslava Kačániová
Department of Microbiology
Faculty of Biotechnology and Food Sciences
Slovak University of Agriculture
Tr. Andreja Hlinku 2
Nitra 949 76, Slovak Republic
Tel: 421-376-414-49
E-mail:[email protected]
Received April 30, 2015; Accepted June 16, 2015; Published June 22, 2015
Citation:Kačániová M, Kluz M, Petrová J, Mellen M, Kunová S, et al. (2015) Incidence of Listeria monocytogenes in Meat Product Samples by Real-Time PCR. Mod Chem appl 3:155. doi: 10.4172/2329-6798.1000155
Copyright: ©2015 Kačániová M et al., This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The aim of this study was to trace a contamination of meat products with Listeria monocytogenes. Step One real time Polymerase Chain Reaction (PCR) was used. We used the PrepSEQ Rapid Spin Sample Preparation Kit for isolation of DNA and MicroSEQ® Listeria monocytogenes Detection Kit for the real-time PCR performance. We found out the strains of Listeria monocytogenes in hundred samples of meat products with no incubation. There was Internal Positive Control (IPC) in 40 samples. Our results showed that the real time PCR assay tested in this study might detect Listeria monocytogenes in meat product samples with no incubation sensitively.

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