alexa Incidence of White Spot Syndrome Virus (Wssv) in Indian Farmed Frozen Shrimp Products and Testing for Viability Through Bio-Inoculation Studies
ISSN: 2155-9546

Journal of Aquaculture Research & Development
Open Access

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Research Article

Incidence of White Spot Syndrome Virus (Wssv) in Indian Farmed Frozen Shrimp Products and Testing for Viability Through Bio-Inoculation Studies

Devivaraprasad Reddy A, Jeyasekaran G* and Jeya Shakila R

Department of Fish Processing Technology, Fisheries College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tuticorin 628 008, India

*Corresponding Author:
Dr. Jeyasekaran G
Department of Fish Processing Technology
Fisheries College and Research Institute
Tamil Nadu Veterinary and Animal Sciences University
Tuticorin 628 008, India
Tel: +91 461 2332354
Fax: +91 461 2340574
E-mail: [email protected]

Received Date: October 04, 2010; Accepted Date: October 22, 2010; Published Date: October 25, 2010

Citation: Reddy AD, Jeyasekaran G, Shakila JR (2010) Incidence of White Spot Syndrome Virus (Wssv) in Indian Farmed Frozen Shrimp Products and Testing for Viability Through Bio-Inoculation Studies. J Aquac Res Development 1:102. doi:10.4172/2155-9546.1000102

Copyright: © 2010 Reddy AD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The incidence of white spot syndrome virus (WSSV) in the Indian farmed frozen shrimp products viz., whole, headless (HL) and peeled and undeveined (PUD) by polymerase chain reaction (PCR) was studied. The viability of WSSV in farmed frozen shrimps was investigated by conducting bio-inoculation studies. The products were screened for WSSV by both the single step (for the primer sets 1s5 & 1a16 and IK1 & IK2) and nested PCR (for the primers IK1 & IK2 - IK3 & IK4). Single step PCR detected WSSV in 18% of samples, while the nested PCR detected WSSV in 73% of samples. The frozen material, which gave positive results for WSSV by PCR, was further confirmed for the viability of WSSV by the bio-inoculation studies. Mortality (100%) was observed within the 45 h of intra-muscular post injection (P.I) into the healthy wild live WSSV-free shrimps (Penaeus monodon). These results show that the WSSV survived the freezing process and the cold storage and could cause infection in native shrimp farms if such products are reprocessed in the importing countries.

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