alexa Increased Myeloperoxidase Expression is Associated with Increase in Intra-Plaque Hemorrhage, Iron Content, Inflammation and Neovascularization in Diabetic Atherosclerosis: Implications for Plaque Progression
ISSN: 2329-9517

Journal of Cardiovascular Diseases & Diagnosis
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Research Article

Increased Myeloperoxidase Expression is Associated with Increase in Intra-Plaque Hemorrhage, Iron Content, Inflammation and Neovascularization in Diabetic Atherosclerosis: Implications for Plaque Progression

Raman Purushothaman1* Carlos L. Alviar2Arthur Tarricone3Prakash Krishnan4Annapoorna Kini5Samin Sharma6Valentin Fuster7Spain8Pedro. R. Moreno9
1Assistant Professor of Medicine/Cardiology/Pathology, The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, USA
2Dept of Internal Medicine, Langone Medical Center, New York University, New York, USA
3Clinical Research Coordinator, The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, USA
4Assistant Professor of Medicine/Cardiology, Director of Endovascular Services, The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, USA
5Professor of Medicine/Cardiology, Director of Interventional Cardiology, The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, USA
6Professor of Medicine/Cardiology, Director of Clinical Cardiology, Dean of International Clinical Affiliation, The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York
7Professor of Medicine and Chairman of Cardiology, The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, USA
8Director, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain
9Professor of Medicine/Cardiology, Director of Cardiovascular Translational Research, The Zena and Michael A. Wiener Cardiovascular Institute, Icahn School of Medicine at Mount Sinai, New York, USA
Corresponding Author : Purushothaman K. Raman
Assistant Professor of Medicine/Cardiology/Pathology
Icahn School of Medicine at Mount Sinai
One Gustave L. Levy Place, Box 1030
New York, NY 10029 USA
Fax: (212) 241-9782
Tel: (212) 241-368
E-mail: [email protected]
Received June 26, 2013; Accepted August 08, 2013; Published August 15, 2013
Citation: Purushothaman R, Purushothaman M, Alviar CL, Tarricone A, Vasquez M, et al. (2013) Increased Myeloperoxidase Expression is Associated with Increase in Intra-Plaque Hemorrhage, Iron Content, Inflammation and Neovascularization in Diabetic Atherosclerosis: Implications for Plaque Progression. J Cardiovasc Dis Diagn 1:118. doi: 10.4172/2329-9517.1000118
Copyright: © 2013 Purushothaman R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Background: Myeloperoxidase (MPO), a heme peroxidase enzyme produced by macrophages, is a marker of oxidative stress associated with increased cardiovascular events in Diabetes Mellitus (DM). In this study we hypothesize that MPO protein and gene expression is associated with increased Intra-Plaque Hemorrhage (IPH), iron deposition, plaque inflammation and neovascularization in DM atheroma.

Methods: Twenty-six human aortas with advanced plaques (14 DM vs.12 No-DM) were studied. MPO protein and gene expression were quantified with immunohistochemistry and RT-PCR respectively. IPH was evaluated using H&E stain. Plaque iron was scored using Perl’s stain and graded Inflammatory cells and neovessels were manually counted in parallel sections using with (CD68 and CD3), and (CD34) using immunohistochemistry method.

Results: Total plaque MPO protein expression density (973.13 ± 28.32 vs. 356.24 ± 26.95; p = 0.0001) and MPO gene expression (0.00267 ± 0.0007 vs. 0.00033 ± 0.0001; p = 0.0001), IPH percentage (57.1% vs. 16.7 %; p= 0.04), iron grade (0.79 ± 0.21 vs. 0.25 ± 0.13; p = 0.05), inflammatory cell grade (1.71 ± 0.10 vs. 1.17 ± 0.11; p = 0.001) and total neovessel content (48.96 ± 4.68 vs. 21.54 ± 2.27; p = 0.0001) were significantly increased in DM plaques when compared to no-DM.

Conclusion: Increased MPO protein and gene expression are associated with a parallel increase in IPH, iron grade, inflammation grade and neovessel content in DM. This suggests a possible role of MPO in enhancing the IPH, iron deposition; inflammation and neovascularization in high risk DM plaques may possibly due to oxidative stress.

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