Induced Pluripotent and Mesenchymal Stem Cells as a Promising Tool for Articular Cartilage Regeneration
Tomasz Trzeciak*, Ewelina Augustyniak, Magdalena Richter, Jacek Kaczmarczyk and Wiktoria Suchorska
Department of Orthopaedics and Traumatology, Poznan University of Medical Sciences, Poland
- *Corresponding Author:
- Tomasz Trzeciak
Department of Orthopaedics and Traumatology
Poznan University of Medical Sciences
28 czerwca Street No 135/147-61 545 Poznan, Poland
Tel: +48 618310 359
Fax: +48 618310 163
E-mail: [email protected]
Received Date: July 24, 2014; Accepted Date: August 25, 2014; Published Date: August 27, 2014
Citation: Trzeciak T, Ewelina A, Magdalena R, Jacek K, Wiktoria S (2014) Induced Pluripotent and Mesenchymal Stem Cells as a Promising Tool for Articular Cartilage Regeneration. J Cell Sci Ther 5:172. doi: 10.4172/2157-7013.1000172
Copyright: © 2014 Trzeciak T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The application of stem cells in regenerative medicine has recently become a rapidly growing field, holding promise for combating a number of orthopedic disorders including osteodegenerative ones (osteoporosis and osteoarthritis). Although the differentiation of stem cells into chondrocytes is now intensively investigated on a laboratory scale, implementing the laboratory protocols in clinical practice requires a scale-up culture. In order to apply this technique many aspects of stem cell bioprocessing such as optimal culture conditions for anchoragedependent or anchorage-independent cells and the type of culture must be taken into account. The presence of microcarriers and/or scaffolds for adherent cells is essential, since they provide a three-dimensional microenvironment indispensable for cell growth. For treatment of osteoarthritis, induced pluripotent stem cells and mesenchymal stem cells seem to be the best choice. Although, the scale-up culture using stem cells has been intensively investigated on a laboratory scale, the scale-up culture for clinical application still requires further technical improvements.In this review stem cell bioprocessing including the use of biomaterials, bioreactors, and factors affecting this process, as well as scale-up culture of induced Pluripotent and mesenchymal stem cells were presented and discussed.