alexa Induced Regenerative Elastic Matrix Repair in LOXL1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse | OMICS International | Abstract
ISSN: 2157-7552

Journal of Tissue Science & Engineering
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Research Article

Induced Regenerative Elastic Matrix Repair in LOXL1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse

Ramamurthi A1*, Venkataraman L1,3, Lenis AT1, Couri BM1 and Damaser MS1,2
1Department of Biomedical Engineering, Cleveland Clinic, Cleveland, OH, USA
2Advanced Platform Technology Center, Louis Stokes Cleveland VA Medical Center, Cleveland, OH, USA
3Department of Bioengineering, Clemson University, USA
Corresponding Author : Ramamurthi A
Department of Biomedical Engineering
Cleveland Clinic, Cleveland, OH, USA
Fax: 216-444-9198
E-mail: [email protected]
Received August 20, 2012; Accepted September 25, 2012; Published September 28, 2012
Citation: Ramamurthi A, Venkataraman L, Lenis AT, Couri BM, Damaser MS (2012) Induced Regenerative Elastic Matrix Repair in LOXL1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse. J Tissue Sci Eng 3:120. doi: 10.4172/2157-7552.1000120
Copyright: © 2012 Ramamurthi A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Impaired elastic matrix remodeling occurs in reproductive tissues, after vaginal delivery. This has been linked to development of pelvic organ prolapse (POP), for which there currently is no pharmacologic therapy. Hyaluronan oligomers and transforming growth factor beta 1 (termed elastogenic factors, EFs), have been shown to significantly enhance tropoelastin synthesis, elastic fiber assembly, and crosslinking by adult vascular smooth muscle cells(SMCs). The goal of this study was to ascertain, if these factors similarly improve the quantity and quality of elastic matrix deposition by vaginal SMCs (VSMCs) isolated from lysyl oxidase, like–1 knock out (LOXL1 KO) mouse model of POP. Cells isolated from whole vagina of a LOXL1 KO mouse (multiparous, stage 3 prolapse) were cultured and identified as SMCs, by their expression of various SMC markers. Passage 2 vaginal SMCs (VSMCs; 3×104/10 cm2) were cultured for 21 days, with EFs. Cell layers and spent medium aliquots were assessed for elastin content and quality. EF-treated VSMCs proliferated at a similar rate to untreated controls, but synthesized more total elastin, primarily in the form of soluble matrix elastin. Elastin mRNA was also increased compared to controls. The elastic matrix was significantly denser in EF-treated cultures, which was composed of more mature, non-interrupted elastic fibers that were absent in controls. The results are promising towards development of a therapy, to enhance regenerative elastic matrix repair in post–partum female pelvic floor tissues.

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