Inflammatory and Cell Adhesion Protein Markers in Colorectal Cancer in Patients Seen at the Teaching Hospital of Guadeloupe: Role of NF kappaB and P-SelectinMonique Decastel1*, Anne-marie Andrea2, Marc Lubeth3, Jacqueline Deloumeaux4 and Juliette Smith-ravin5
- *Corresponding Author:
- Monique Decastel
UMR Inserm_S1134, CNRS SNC 9169
CHU de Pointe-à-Pitre, bâtiment Ricou
97159 Guadeloupe, France
Tel: +33 (0)590 83 48 99
Fax: +33 (0)590 83 05 13
E-mail: [email protected]; [email protected]
Received Date: June 17, 2014; Accepted Date: August 29, 2014; Published Date: September 01, 2014
Citation: Decastel M, Andrea A, Lubeth M, Deloumeaux J, Smith-ravin J (2014) Inflammatory and Cell Adhesion Protein Markers in Colorectal Cancer in Patients Seen at the Teaching Hospital of Guadeloupe: Role of NF kappaB and P-Selectin. J Cytol Histol 5:276. doi:10.4172/2157-7099.1000276
Copyright: © 2014 Decastel M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Previous reports have been published describing the role of protein markers in the development and progression of colorectal cancers (CRC). In those investigations, Nuclear Factor kappaB (NFκB) and the Hypoxia Inducible Factor-1α (HIF-1α) have emerged as markers of clinical interest. The aim of this study was to evaluate their expression in patients who underwent surgery for CRC in the Guadeloupe Teaching Hospital (GlpeTH).
Methods: Tumour tissues and the normal mucosa from 67 patients of GlpeTH were immunohistochemically evaluated using antibodies against HIF-1α and NFκBp65. Additionally; we examined the expression of Vascular Endothelial Growth factor (VEGF), Vascular Cell Adhesion Molecule-1 (VCAM-1) and P-selectin, which was regulated by HIF-1α and NFκBp65, respectively.
Results: Expression of NFκBp65 and HIF-1α, mainly located in the cytoplasm of the cancer cells and P-selectin, located on endothelial cells of blood vessels, was positively observed in 74.6%, 73.1% and 23.9% among the patients, respectively. Unexpectedly, expression of VEGF and VCAM-1 was weak, 6% and 3% respectively. HIF-1α did not associate either with clinicopathological parameters or NFκBp65 expression, whereas the latter significantly did with invasion depth (p=0.017). Regarding P-selectin, its expression was not correlated with that of NFκBp65, but significantly was with TNM stage (p=0.022).
Conclusion: Expression of VEGF and VCAM-1 may depend of other tumour environmental factors. NFκB, more frequently expressed in T3-T4 stages and P-selectin less in aggressive CRCs (TNM III and IV) are more valuable protein markers than HIF-1α, to characterize CRC in GlpeTH patients.