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In-Flow DNA Extraction Using on-Chip Microfluidic Amino-Coated Silicon Micropillar Array Filter | OMICS International | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
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Research Article

In-Flow DNA Extraction Using on-Chip Microfluidic Amino-Coated Silicon Micropillar Array Filter

Hanaa M Hegab1,2,3,4*, Soliman M3, Ebrahim S3 and Op de Beeck M2

1KACST-Intel Consortium Center of Excellence in Nano-manufacturing Applications (CENA), Riyadh, Saudi Arabia

2Imec, Kapeldreef 75, Leuven, Belgium

3Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt

4Institute of Advanced Technology and New Materials, City of Scientific Research andTechnological Applications, Borg Elarab, Alexandria, Egypt

*Corresponding Author:
Hanaa M Hegab
IMEC, Kapeldreef 75
Leuven, Belgium
Tel: +32488694814
E-mail: [email protected]

Received Date: July 28, 2013; Accepted Date: August 29, 2013; Published Date: September 05, 2013

Citation: Hegab HM, Soliman M, Ebrahim S, Op de Beeck M (2013) In-Flow DNA Extraction Using on-Chip Microfluidic Amino-Coated Silicon Micropillar Array Filter. J Biosens Bioelectron 4:140. doi: 10.4172/2155-6210.1000140

Copyright: © 2013 Hegab HM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A silicon micropillar filter (μPF) chip was fabricated, glass-bonded, coated with 3-aminopropyltriethoxysilane (APTES) then tested for its ability to bind and release pre-purified DNA in continuous flow mode. APTES coated μPF was tested over different DNA initial loading amounts and flow rates. Simple and effective washing and eluting steps were used, which allowed direct detection of obtained DNA using UV Nano-Droplet. Both the capture efficiency and elution % were inversely proportional to flow rate, with such relation being more profound at higher DNA concentration. DNA elution recovery %, as high as 80%, was achieved under flow rate of 5 μl /min and an initial DNA loading of 250 ng. The capture capacity per surface area of the APTES-coated μPFis ~186 ng/cm2, which mounts to ~3.7 times the capacity of an identical uncoated filter. These results demonstrate the potential for integrating such amino-coated μPF in a lab-on-a-chip system for the extraction of nucleic acids directly from clinical samples in a continuous flow mode.

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