Abstract

Objective: To explore the effect of allogenic bone marrow transplant on cardiac function in rats after AMI.
Material and methods: 50 Male, Wistar rats were randomly divided into transplant group and control 1, 2, 3 groups. Acute myocardial infarction (AMI) model was designed by ligation of the left anterior descending (LAD) coronary artery. Transplant group; prepared allogeneic marrow mononuclear cells (BM-MNCs) suspension were implanted in the epicardial area around the infarcted region. Control group 1; simple thoracotomy without ligation of coronary arteries. Control group 2; only AMI model was established. Control group 3; culture medium was table implanted into the subepicardioial and peri-infarction area. 4 weeks after transplantation, echocardiography and cardiac catheterization assessment of left ventricular morphology as well as function were performed. Morphological characteristics and organization of transplanted BM-MNCs within the infarcted area and its periphery was observed.
Results: Compared with control group 1, left ventricular systolic diameter (LVEDs), left ventricular end-diastolic diameter (LVEDd) and left ventricular end-diastolic pressure (LVEDP) were significantly increased (P <0.05), while left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS), left ventricular systolic pressure (LVSP), maximal rate of left ventricular pressure (+ dp/dt max) and maximal decrease rate left ventricular pressure (-dp / dtmax) were significantly lower (P <0.05) in control group 2, 3 and transplantation group; LVEDs, LVEDd and LVEDP in transplantation group were significantly lower (all P <0.05); while LVEF, LVFS, + dp/dt max and -dp/dt max were significantly higher than the control group 2 and 3 (P <0.05). Immunohistochemistry showed that BrdU labeling BM-MNCs were survived in the transplantation group.
Conclusion: Allogenic BM-MNCs transplantation in rat can reduce the left ventricular dilatation, enhance inhibition of left ventricular remodeling, and improve cardiac function after AMI.