alexa Influence of Demethylation Pathway on the Differentiation Potential of Human Mesenchymal Stem Cells | OMICS International | Abstract
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
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Research Article

Influence of Demethylation Pathway on the Differentiation Potential of Human Mesenchymal Stem Cells

Faisal Ali1,2*, Yazan Ranneh1, Amin Ismail1 and Bart Vaes2

1Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Nutrigenomics Programme, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

2Metabolism and genomics group, Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands

*Corresponding Author:
Faisal Ali
Department of Nutrition and Dietetics
Faculty of Medicine and Health Sciences
Nutrigenomics Programme, Universiti Putra Malaysia
43400 UPM Serdang, Selangor, Malaysia
Tel: +60172217485
Fax: +60389426769
E-mail: [email protected]

Received date: September 03, 2013; Accepted date: September 21, 2013; Published date: September 23, 2013

Citation: Ali F, Ranneh Y, Ismail A, Vaes B (2013) Influence of Demethylation Pathway on the Differentiation Potential of Human Mesenchymal Stem Cells. J Stem Cell Res Ther 3:153. doi:10.4172/2157-7633.1000153

Copyright: © 2013 Ali F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Osteoporosis is associated with a decrease in the commitment of Mesenchymal stem cells (MSC) to the bone forming osteoblast lineage and an increase in the commitment to the fat forming adipocyte lineage in bone marrow of elderly persons. A link between methylation pathway and MSC differentiation remains unclear. Therefore, we hypothesize that alterations in the commitment and differentiation potential of MSC during osteoporosis may be mediated by modification of global methylation pathway. To examine the role of methylation pathway on the differentiation potential of MSC into osteoblasts or adipocytes, human MSC, were used. Adox, which mimics hyperhomocysteinemia effect, was added to cells as a potent methylation inhibitor to block the global methylation pathway of DNA, RNA, Lipid and protein. The effect of demethylation on osteoblast differentiation was determined by measuring the alkaline phosphates activity and the degree of calcification. While, adipocyte differentiation was determined by Oil-red O staining and triglyceride content. It was clearly observed that demethylation reduces alkaline phosphates activity, calcification and thereby, osteoblast differentiation. In contrast, adipocyte differentiation was stimulated by demethylation. The results of this study suggest that demethylation changes the differentiation potential of MSC for more adipogenic and less osteogenic.

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