Influence of Histamine H1 Receptor Antagonists on Thioredoxin Production In vitro and In vivoTomomi Mizuyoshi1, Masayo Asano1, Atsuko Furuta1, Kazuhito Asano2* and Hitome Kobayashi1
- *Corresponding Author:
- Kazuhito Asano
Division of Physiology, School of Nursing and Rehabilitation Sciences
Showa University, 1865 Touka-Ichiba, Midori-ku, Yokohama 226-8555, Japan
Tel: +81 45 985 6538
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E-mail: [email protected]
Received date: May 08, 2017; Accepted date: May 16, 2017; Published date: May 24, 2017
Citation: Mizuyoshi T, Asano M, Furuta A, Asano K, Kobayashi H (2017) Influence of Histamine H1 Receptor Antagonists on Thioredoxin Production In vitro and In vivo. J Allergy Ther 8:258. doi: 10.4172/2155-6121.1000257
Copyright: © 2017 Mizuyoshi T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Thioredoxin (TRX), a 12-kDa oxidoreductase enzyme, is well known to be a redox-active protein that regulates reactive oxidative metabolism. TRX is also accepted to be a protein with anti-inflammatory effects and reported to attenuate the development of allergic airway inflammatory diseases such as allergic rhinitis (AR) and asthma. Although histamine H1 receptor antagonists are frequently used for the treatment of AR, the influence of the agents on TRX production is not well understood. In the present study, we examined the influence of fexofenadine (FEX), cetirizine (CT), and levocetirizine (LCT), which are classified into histamine H1 receptor antagonists, on TRX production in vitro and in vivo.
Methods: Macrophages derived from THP-1 cells (1 × 105 cells/ml) were cultured with 50 μM H2O2 in combination with/without the agents for 24 h. Nasal secretions were obtained from patients with Japanese cedar pollen-sensitized rhinitis, who were treated with FEX or LCT for four weeks during pollen season. TRX contents in both culture supernatants and nasal secretions were examined by ELISA.
Results: Addition of FEX, CT and LCT into macrophage cultures increased TRX levels in supernatants. The minimum concentration of the agents that caused significant increase was 0.3 μM for FEX, 0.4 μM for CT and LCT. Treatment of patients with FEX and LCT also caused increase in TRX levels in nasal secretions along with attenuation of clinical symptoms.
Conclusion: Histamine H1 receptor antagonists may increase the ability of macrophages to produce TRX, and results in favorable modification of clinical conditions of AR.