INFLUENCE OF HORMONAL TREATMENTS AND OF SUCROSE ON THE MICROTUBERIZATION OF THREE POTATO VARIETIES (Solanum tuberosum L.) ADAPTED TO AGROCLIMATIC CONDITIONS IN SENEGAL
|DIEMEAbraham, SAGNAMaurice, SY Mame Ourèye
Laboratoire Campus de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences & Techniques, Université Cheikh Anta Diop, BP 5005, Dakar - Fann, Sénégal
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In the situation of a research-development project on the in vitro production of potato seeds in Senegal, we attempted to improve the microtuberization of some varieties. The production of vitrotubers is one of the most efficient tools for the propagation of basic material, the transport of germplasm and the conservation of potato varieties cultivated after cleansing. It allows, at any period of the year, to obtain healthy seeds ready to be planted and which can be dispatched within long distances without any specific precaution. In this context, comparative studies have been achieved under darkness conditions and at 20°C, on two types of Murashige & Skoog (MS/2) media of tuberization, with, on the one hand, a sucrose concentration of 80 or 100 g L-1, without hormones and on the other with different hormonal combinations (Kinetin + BAP) or (Kinetin + Coumarin). Aïda variety presents the best aptitude for the microtuberization with a rate of 91%. The sucrose concentration most favorable being 80 g L-1 for the Aïda variety and 100 g L -1 for the Atlas and Odessa varieties. The Aïda variety (88.5%) gave more microtubers when the hormonal combination Kinetin + BAP is added to the culture medium, compared to the Odessa (74.5%) and Atlas (66.5%) varieties. The replacement of the BAP by Coumarin in the hormonal combination was not beneficial. We noticed, indeed, a reduction of the microtuberization rate for all varieties whatever was the sucrose concentration added to the culture medium. The output in mass of the microtubers depends as well on the caliber and the number of microtubers.