alexa Inhibition of Alpha-Glucosidase by Acacia nilotica Prevents Hyperglycemia along with Improvement of Diabetic Complications via Aldose Reductase Inhibition | Abstract
ISSN: 2155-6156

Journal of Diabetes & Metabolism
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Research Article

Inhibition of Alpha-Glucosidase by Acacia nilotica Prevents Hyperglycemia along with Improvement of Diabetic Complications via Aldose Reductase Inhibition

Natasha Jaiswal1, Swayam P Srivastava1, Vikram Bhatia1, Akansha Mishra1, Amit K Sonkar2, Tadigoppula Narender3, Arvind K Srivastava1 and Akhilesh K Tamrakar1*

1Division of Biochemistry, CSIR-Central Drug Research Institute, India

2Division of Molecular and Structural Biology, CSIR-Central Drug Research Institute, India

3Division of Medicinal Process Chemistry, CSIR-Central Drug Research Institute, India

*Corresponding Author:
Akhilesh K Tamrakar
Division of Biochemistry
Central Drug Research Institute
CDRI Communication No. 8319
Lucknow-226001, India
Tel: +91-0522-2212411-18
Fax: +91-0522-2223938
E-mail: [email protected]

Received date: August 01, 2012; Accepted date: September 07, 2012; Published date: September 12, 2012

Citation: Jaiswal N, Srivastava SP, Bhatia V, Mishra A, Sonkar AK, et al. (2012) Inhibition of Alpha-Glucosidase by Acacia nilotica Prevents Hyperglycemia along with Improvement of Diabetic Complications via Aldose Reductase Inhibition. J Diabetes Metab S6:004. doi:10.4172/2155-6156.S6-004

Copyright: © 2012 Jaiswal N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Abstract

Postprandial hyperglycemia is a prominent and early defect in diabetes and regulating blood glucose elevation may attenuate progression towards diabetes associated secondary complications. Here we investigated the alphaglucosidase inhibitory potential of the ethanolic extract of the stem bark of Acacia nilotica (EEAN). The EEAN showed a remarkable alpha-glucosidase inhibitory effect with IC50 value around 8 μg/ml. Kinetic studies revealed that the extract inhibited alpha-glucosidase in competitive manner and caused conformational changes in secondary structure of the enzyme protein. In vivo analysis showed that EEAN significantly suppresses the sucrose-induced postprandial glucose elevation in normal rats and exerts antihyperglycemic effect in streptozotocin (STZ)-induced diabetic rats in a dose-dependent fashion. Further, treatment of diabetic animals after 10 week of STZ-treatment with EEAN (250 mg/ kg) for 21 days, significantly reduced the elevated levels of blood glucose, %HbA1C, urea, uric acid and creatinine, and significantly increased the depressed plasma insulin level. The EEAN also showed inhibitory potential on aldose reductase activity with an IC50 of 7.5 μg/ml. The results suggest that EEAN possess antihyperglycemic activity through inhibition of alpha-glucosidase along with antidiabetogenic effect on polyol pathway through aldose reductase inhibition.

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