Inhibitory Effects of Hybrid Liposomes on the Growth of Non-small Cell Lung Carcinoma Cells and Anti-invasive Activity by Ceramide Generation without any DrugsHideaki Ichihara, Yuji Komizu, Ryuichi Ueoka and Yoko Matsumoto*
Division of Applied Life Science, Graduate School of Engineering, Sojo University, Ikeda, Nishi-ku, Kumamoto, Japan.
- *Corresponding Author:
- Yoko Matsumoto
Division of Applied Life Science
Graduate School of Engineering, Sojo University
4-22-1 Ikeda, Nishi-ku, Kumamoto 860-0082, Japan
Tel +81 96 326 3965
Fax +81 96 323 1311
E-mail: [email protected]
Received date: June 08, 2015 Accepted date: July 03, 2015 Published date: July 06, 2015
Citation: Ichihara H, Komizu Y, Ueoka R, Matsumoto Y (2015) Inhibitory Effects of Hybrid Liposomes on the Growth of Non-small Cell Lung Carcinoma Cells and Anti-invasive Activity by Ceramide Generation without any Drugs. J Carcinog Mutagen 6:230. doi: 10.4172/2157-2518.1000230
Copyright: © 2015 Ichihara H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ceramide generation and invasive inhibition by hybrid liposomes (HL) composed of 90 mol% L-α-dimyristoylphosphatidylcholine (DMPC) and 10 mol% polyoxyethylene (23) dodecyl ether (C12(EO)23) were examined for human lung cancer (A549) cells in vitro. Ceramide generation for A549 cells treated with HL was observed through the activation of neutral sphingomyelinase. These was a reduction in sphingomyelin in A549 cells after treatment with HL. The anti-invasive effects of HL for A549 cells were obtained through the inhibition of MT1MMP/MMP14. Inhibitory effects of HL on the migration of A549 cells was obtained using scratch assay.