Molecular Detection of Hepatitis B Virus DNA in Human Immunodeficiency Virus Infected Patients in Khartoum, SudanYassin Elfaki1*, Khalid A Enan2, Isam M Elkhidir3and Abdelbagi M Nagi1
- *Corresponding Author:
- Yassin Elfaki
Department of Experimental Immunology
Helmholtz Centre for Infection Research
Inhoffenstr- 7, 38124 Braunschweig, Germany
Tel: +49 (0) 40 7410 -58731
E-mail: [email protected]
Received date: December 29, 2016; Accepted date: January 11, 2017; Published date: January 18, 2017
Citation: Elfaki Y, Enan KA, Elkhidir IM, Nagi AM (2017) Molecular Detection of Hepatitis B Virus DNA in Human Immunodeficiency Virus Infected Patients in Khartoum, Sudan. Mol Biol 6:184. doi:10.4172/2168-9547.1000184
Copyright: © 2017 Elfaki Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Since hepatitis B virus (HBV) and human immunodeficiency virus (HIV) have the same modes of transmission, it is usual for them to infect the same host. This study was conducted at Bashair University Hospital during the period from April to July 2013, to detect HBV among HIV-infected individuals using polymerase chain reaction (PCR). Methodology: A total of 87 subjects (n=87) were included in this observational, descriptive, case-study. The subjects were confirmed as HIV-positive by an ELISA assay, from ages ranged from 16 to 60 years, and from both sexes. From the study participants, serum samples were collected and tested for HBsAg by a capture ELISA assay and for HBV DNA by PCR. Results: Out of the 87 people who took part in the study, 13 (14.9%) were positive for HBV DNA, while 14 (16.1%) were positive for HBsAg. Six people (6.9%) tested positive for both HBV DNA and HBsAg and 7 people (8.0%) were found to have occult hepatitis B infection (OBI) (i.e., positive for HBV DNA, negative for HBsAg). Conclusion: From the above findings we concluded that, there is a high percentage of HBV/HIV coinfection in the Sudan. Also, there is an increasing percentage of OBI in HIV patients. Hence, we recommend the screening of HIVpositive subjects for HBV markers and the use of HBV DNA as a marker of OBI in the same population.