alexa Interferon-γ Upregulates SOCS3 Expression to Red
ISSN: 2167-7700

Chemotherapy: Open Access
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Research Article

Interferon-γ Upregulates SOCS3 Expression to Reduce Cisplatin Chemoresistance in Non-Small Cell Lung Cancer A549 Cells

Xu Y1,2, Tang H2, Gong L1, Hu H1 , Chen L1,2, Sui X1,2, Xia D3 and Pan H1,2*

1Department of Medical Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China

2Biomedical Research Center and Key Laboratory of Biotherapy of Zhejiang Province, College of Medicine, Zhejiang University, Hangzhou, China

3School of Public Health, College of Medicine, Zhejiang University, Hangzhou, China

*Corresponding Author:
Hongming Pan
Biomedical Research Center and Key Laboratory of Biotherapy of Zhejiang Province
College of Medicine
Zhejiang University
Hangzhou, China
Tel: 86 0571 8600 6926
E-mail: [email protected]

Received date: April 20, 2016; Accepted date: June 04, 2016; Published date: June 09, 2016

Citation:Xu Y, Tang H, Gong L, Hu H, Chen L, et al. (2016) Interferon-γ Upregulates SOCS3 Expression to Reduce Cisplatin Chemoresistance in Non-Small Cell Lung Cancer A549 Cells. Chemo Open Access 5: 205. doi:10.4172/2167-7700.1000205

Copyright: © 2016 Xu Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Abstract Background: Cisplatin (DDP)-based chemotherapy is the mainstay of first-line therapeutic strategy for the treatment of advanced non-small cell carcinoma (NSCLC). However, the anticancer efficacy of DDP is often limited by the existence or development of chemoresistance. Thus, we investigated the effect of interferon-γ on DDPresistant A549 cell. Methods: Semi-quantitative RT–PCR was used to compare the differences of SOCS3 mRNA expression in both cisplatin-resistant A549 (A549/DDP) cell and the parental A549 cell. The cellular sensitivity to cisplatin, cell viability and apoptosis were detected by MTT, flow cytometry and Western blotting. Results: Semi-quantitative RT–PCR and western blotting showed that SOCS 3 expression was significantly down-regulated in A549/DDP cell compared to the parental A549 and normal human bronchial epithelial cells BEAS-2B. IFN-γ treatment could restore SOCS3 expression, resulting in an increased sensitivity of these resistant A549 cells to DDP. In addition, p53 and Bcl-2 signaling pathways were also involved in regulating IFN-γ-induced cell death in DDP-resistant A549 cells. Conclusion: Our study indicates that SOCS 3 may play a crucial role in the progress of cisplatin resistance of A549. Moreover, IFN-γ could induce SOCS3 expression and potentiate the re-sensitization of these resistant A549 cells to DDP

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