Interleukin 1-ÃÂ±: A Modulator of Melanocyte Homeostasis in Vitiligo
Mala Singh, Mohmmad Shoab Mansuri, Mansi A Parasrampuria and Rasheedunnisa Begum*
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat -390002, India
- *Corresponding Author:
- Rasheedunnisa Begum
Department of Biochemistry
Faculty of Science
The Maharaja Sayajirao University of Baroda, Vadodara
Tel: +91 265-2795594
E-mail: [email protected]
Received date: February 05, 2016; Accepted date: May 12, 2016; Published date: May 16, 2016
Citation: Singh M, Mansuri MS, Parasrampuria MA, Begum R (2016) Interleukin 1-α: A Modulator of Melanocyte Homeostasis in Vitiligo. Biochem Anal Biochem 5: 273. doi:10.4172/2161-1009.1000273
Copyright: © 2016 Singh M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Vitiligo is a hypomelanotic autoimmune skin disorder arising from a breakdown in immunological self-tolerance, which leads to aberrant immune responses against melanocytes leading to selective destruction of melanocytes. High levels of Interleukin 1(IL1) have been reported in various autoimmune disorders including vitiligo. The aim of present study was to explore the effect of IL1-α on melanocyte biology by monitoring the melanocyte viability, IL1R1 membrane expression, melanogenesis (TYR, TYRP1 and MITF-M) and other immuno modulatory molecules (IL1A, IL1B, IL1RN,IL1R1 IL8, TNFA, IL6, and ICAM1) upon exogenous stimulation of IL1- α on primary cultured normal human melanocytes (NHM).
Materials and methods: Melanocytes were isolated from normal c skin biopsies and cultured in vitro. The NHMs were treated with IL1-α (0-100 ng/ml) and cell viability was monitored by MTT assay, IL1R1 membrane expression was monitored using flow cytometry and relative gene expression was performed using semi-quantitative RT-PCR.
Results: The dose dependent effect of IL1-α on melanocytes showed ~12% melanocyte death and ~22% increase in IL1R1 membrane expression upon 100 ng/ml IL1-α treatment for 48 hrs. Further, IL1RN, IL1A, IL1B, IL6, TNFA, ICAM1 showed significantly increased expression (p<0.05) and MITF-M showed significantly decreased expression (p<0.05) upon IL1-α (10 and 100 ng/ml, 48 hrs) stimulation on NHM; while TYR, TYRP1, IL8 and IL1R1 showed no difference.
Conclusion: Overall, the present study suggests the crucial role of IL1-α in melanocyte destruction in vitiligo by regulating MITF-M and other immunomodulatory molecules.