Introducing of a New Experimental Method in Semen Preparation: Supernatant Product of Adipose Tissue: Derived Mesenchymal Stem Cells (SPAS)
|Hoda Fazaeli, Davoodi Faeze, Kalhor Naser, Safarloo Maryam, Masaii Mohammad, Ghiasi Mahdieh and Tabatabaii Qomi Reza*|
|Infertility treatment center of academic center of education, culture and research (ACECR), Qom branch, Iran|
|Corresponding Author :||Tabatabaii Qomi Reza
Infertility treatment center of academic center of education
culture and research (ACECR), Qom branch, Iran
E-mail: [email protected]
|Received March 02, 2016; Accepted March 27, 2016; Published April 03, 2016|
|Citation: Fazaeli H, Faeze D, Naser K, Maryam S, Mohammad M, et al. (2016) Introducing of a New Experimental Method in Semen Preparation: Supernatant Product of Adipose Tissue: Derived Mesenchymal Stem Cells (SPAS). The Patient’s Perspective. J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol 4:178. doi:10.4172/2375-4508.1000178|
|Copyright: © 2016 Fazaeli H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Human male infertility mostly is due to deficient semen parameters. One of the seminal plasma components providing chemical function of the ejaculate is growth factors functioning as paracrine, autocrine, and/or endocrine regulators of cell growth and differentiation. Adipose tissue-derived mesenchymal stem cells secret several proteins, such as cytokine, growth factors, and chemokine. On the other hand, due to contemporary semen preparation methods limitations, development of a new approach having least adverse effects and most efficiency is unavoidable. So, we decided to assess the effect of semen sample incubation with SPAS on semen parameters including motility, viability and DNA fragmentation.
Semen sample of infertile men attending infertility treatment center whose were 30-40 years old was incubated with supernatant product of adipose tissue- derived mesenchymal Stem cells (SPAS). Motility parameters, viability and DNA fragmentation were evaluated using CASA software, eosin- nigrosine staining and halosperm kit respectively. Then conventional method of semen preparation for Intra Uterine Insemination (IUI)- Density gradient centrifugation (DGC)- was compared with SPAS method.
Incubation of semen samples with SPAS (specially SP of 8×105 AD-MSCs for 40 minutes at 1:1 ratio) significantly increased motility parameters while decreased DNA fragmentation (p≤0.05) and has no effect in terms of semen viability. Also, SPAS was significantly more influential in improvement of motility parameters than DGC (p≤0.05).
Since effect of growth factors in improvement of motility and DNA fragmentation is documented in previous studies, it seems that SPAS can be an effective method which provides all these factors together.