Journal of Nutrition & Food Sciences
Open Access
ISSN: 2155-9600
ISSN: 2155-9600
Rosa Martha Perez Gutierrez and Diana Madrigalez Ahuatzi
Aims: The role of free radical reactions in disease pathology is well established, suggesting that these reactions are necessary for normal metabolism but can be detrimental to health as well acting on different levels in an oxidative sequences, the antioxidants prevents the risk of several aging related diseases including cancer, cardio vascular disorder, diabetes, neurodegenerative disorders and others. Main methods: Bioactivity-guided fractionation of a hexane extract of the seeds of P. canariensis afforded three new diterpenes: Canarien A-C, their structures were established based on interpretation of their MS, as well as 1D and 2D NMR data. In this study, investigating the antioxidant activities of Canarien A-C, which have been evaluated in assay •OH, •O2 −, DPPH•, HOCl•, NO, ONOO•, H2O2, ORAC, SOD, ABTS+-scavenging assays, Fe2+-chelating, lipid peroxidation, inhibition lipoxygenase (LOX) and determination of enzymatic antioxidants activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GSH), and glutathione peroxidase (CSH-Px) in liver.
Key findings: The three diterpenes showed effective Hdonor, free radical scavenging, metal chelating ability, and inhibition of lipid peroxidation compared to standard antioxidants such as BHT, α-tocopherol, curcumin, BHA and quercetin. In addition showed inhibition of lipoxygenase and a moderate increase in the activity of enzymes SOD, CAT, GSH and CSH-Px in liver.
Significance: It may be concluded that hexane extract and diterpenes from P. canariensis seeds acts as an antioxidant and ROS scavenger, may protect against oxidative stress and hepatic damages.