alexa Investigation of Human Mesenchymal Stromal Cells Cultured on PLGA or PLGA/Chitosan Electrospun Nanofibers
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Research Article

Investigation of Human Mesenchymal Stromal Cells Cultured on PLGA or PLGA/Chitosan Electrospun Nanofibers

Fatemeh Ajalloueian1,2, Moa Fransson3, Hossein Tavanai1, Mohammad Massumi4, Jöns Hilborn2, Katarina Leblanc5, Ayyoob Arpanaei6* and Peetra U Magnusson3*
1Department of Textile Engineering, Center of Excellence in Applied Nanotechnology, Isfahan University of Technology, Isfahan, 84156-83111, Iran
2Polymer Chemistry, Department of Chemistry, Ångström Laboratory, SciLife Lab, Uppsala University, 751 21 Uppsala, Sweden
3Department of Immunology, Genetics and Pathology, Uppsala University, 751 85 Uppsala, Sweden
4Department of Physiology, University of Toronto, Toronto, ON M5S 1A8, Canada
5Department of Clinical Immunology and Transfusion Medicine, Karolinska Institutet and Hematology Center at Karolinska University Hospital, Stockholm, Sweden
6Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, 14965-161, Iran
Corresponding Authors : Ayyoob Arpanaei
Department of Industrial and Environmental Biotechnology
National Institute of Genetic Engineering and Biotechnology
Tehran 14965-161, Iran
Tel: 45-452-549-29
E-mail: [email protected]
  Peetra U Magnusson
Department of Immunology, Genetics and Pathology
Uppsala University, 751 85 Uppsala, Sweden
E-mail: [email protected]
Received May 22, 2015; Accepted June 12, 2015; Published June 16, 2015
Citation: Ajalloueian F, Fransson M, Tavanai H, Massumi M, Hilborn J, et al. (2015) Investigation of Human Mesenchymal Stromal Cells Cultured on PLGA or PLGA/Chitosan Electrospun Nanofibers. J Bioprocess Biotech 5:230. doi: 10.4172/2155-9821.1000230
Copyright: © 2015 Ajalloueian F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

We compared the viability, proliferation, and differentiation of human Mesenchymal Stromal Cells (MSC) after culture on poly(lactic-co-glycolic acid) (PLGA) and PLGA/chitosan (PLGA/CH) hybrid scaffolds. We applied conventional and emulsion electrospinning techniques, respectively, for the fabrication of the PLGA and PLGA/ CH scaffolds. Electrospinning under optimum conditions resulted in an average fiber diameter of 166 ± 33 nm for the PLGA/CH and 680 ± 175 nm for the PLGA scaffold. The difference between the tensile strength of the PLGA and PLGA/CH nanofibers was not significant, but PLGA/CH showed a significantly lower tensile modulus and elongation at break. However, it should be noted that the extensibility of the PLGA/CH was higher than that of the nanofibrous scaffolds of pure chitosan. As expected, a higher degree of hydrophilicity was seen with PLGA/ CH, as compared to PLGA alone. The biocompatibility of the PLGA and PLGA/CH scaffolds was compared using MTS assay as well as analysis by scanning electron microscopy and confocal microscopy. The results showed that both scaffold types supported the viability and proliferation of human MSC, with significantly higher rates on PLGA/ CH nanofibers. Nonetheless, an analysis of gene expression of MSC grown on either PLGA or PLGA/CH showed a similar differentiation pattern towards bone, nerve and adipose tissues.

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