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Abstract
Investigation of Ph-Assisted Human Serum Albumin (HSA)-Cobalt (Co)Binding Using Nanomechanical Deflection and Circular Dichroism
Garima Thakur, Seonghwan Kim, Kovur Prashanthi and Thomas Thundat
Human serum albumin (HSA)-cobalt (Co) binding assay could assist in early detection of myocardial ischemia.
However, the binding mechanism of the HSA-Co assay is very complex and hard to detect. During myocardial
ischemia, the physiological pH may change with subsequent decrease in binding of Co with HSA. In this paper, we have investigated the binding of Co with HSA functionalized on the surface of microcantilever at an acidic, basic and neutral pH. Conformational change of HSA upon injection of Co(II) ions as analyteat various pH, resulted in nanomechanical deflection of cantilevers. Furthermore, spectroscopic insight was obtained using circular dichroism to analyze conformational change of HSA-Co(II) binding.