In-vitro Approach of Medicinal Herb: Bacopa monnieri
Bacopa (Brahmi) is a medicinal plant of high commercial potential with legendary reputation as a memory vitalizer. The plant is used in traditional Ayurvedic medicine in India for thousands of years to treat asthma, epilepsy and mental illness. Many different studies have showed that Bacopa is useful in treating Alzheimer’s disease, anxiety, and age-related cognitive decline. It is also used to improve mental function and memory, treat respiratory ailments, and heal stomach ulcers. In this study Nodal segments of Bacopa have been taken which contained axillary buds. These buds were surface sterilized and were inoculated aseptically on culture medium. Axillary bud break was achieved in semisolid MS medium. These proliferated axillary shoots were excised and sub cultured on MS medium supplemented with 1.5 mg/l BAP for shoot multiplication. After shoot multiplication 1.0 mg/l IBA was used for rooting (90% rooting plantlets) and ¼ strength of liquid MS medium having 2% sucrose for hardening and acclimatized in the field. This showed better mass production of Bacopa monnieri.