alexa In-vitro Investigation of Fractionated Extracts of Albizia gummifera Seed Against Leishmania donovani Amastigote Stage
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
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Research Article

In-vitro Investigation of Fractionated Extracts of Albizia gummifera Seed Against Leishmania donovani Amastigote Stage

Dereje Nigussie1*, Geremew Tasew1, Eyasu Makonnen2, Asfaw Debella1, Birhanu Hurrisa1, Kelbessa Urga1 and Adugna Wayessa1
1Ethiopian Public Health Institute, Addis Ababa, Ethiopia
2Department of Pharmacology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
Corresponding Author : Dereje Nigussie
Vaccines and diagnostic Production directorate
Ethiopian Public Health Institute
P.O. Box 1242 Addis Ababa Ethiopia
Tel: +251911660850
E-mail:
[email protected]
Received: October 28, 2015 Accepted: November 18, 2015 Published: December 08, 2015
Citation: Nigussie D, Tasew G, Makonnen E, Debella A, Hurrisa B, et al. (2015) In-vitro Investigation of Fractionated Extracts of Albizia gummifera Seed Against Leishmania donovani Amastigote Stage. J Clin Cell Immunol 6:373. doi:10.4172/2155-9899.1000373
Copyright: © 2015 Nigussie D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Natural products represent a rich and promising source of novel, biologically active chemical entities for treating Leishmaniasis. This study investigates the anti-leishmanial activity of n-butanol, aqueous, and chloroform fractions obtained from crude methanolic extract of Albizia gummifera seed in in-vitro (peritoneal mice macrophage) model after one time treatment. The anti-lesihmanial activity was determined from the minimum inhibitory concentration (MIC), infection rate (IR) and multiplication index (MI) against L. donovani. Cyto-toxicity of the fractions was also assessed against Vero cells. In addition to this secondary metabolites were determined by phytochemical screening. The MIC value of n-butanol, aqueous, chloroform and amphotericin B were 11.2 μg/ml, 33.5 μg/ml, >89 μg/ml and 1.32 μg/ml, respectively. N-butanol and aqueous fractions significantly inhibit the growth of intra-cellular L. donovani amastigote (P>0.05) compared to amphotericin B whereas the chloroform fraction did not revealed any significant leishmanicidal effect (P<0.05) with their respective parasitic infection rate, multiplication index and MIC levels. Based on these data, the n-butanol and aqueous fractionates of A. gummifera seeds exhibit considerable in-vitro anti L. donovani activity and may be promising anti-leishmanial herbal remedy candidate that support the traditional use of the plant.

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