In-Vivo Antidiabetic Activity and Safety of The Aqueous Stem Bark Extract of Kleinia squarrosaAbdirahman YA1*, Juma KK1, Mukundi MJ1,3, Gitahi SM1, Agyirifo DS1, Ngugi MP1, Gathumbi PK2, Ngeranwa JJN1 and Njagi ENM1
- *Corresponding Author:
- Abdirahman YA
Department of Biochemistry and Biotechnology
Kenyatta University, P.O. Box 43844-00100, Nairobi, Kenya
E-mail: [email protected] gmail.com
Received date: August 10, 2015; Accepted date: September 05, 2015; Published date: September 09, 2015
Citation: Abdirahman YA, Juma KK, Mukundi MJ, Gitahi SM, Agyirifo DS, et al. (2015) In-Vivo Antidiabetic Activity and Safety of The Aqueous Stem Bark Extract of Kleinia squarrosa. J Diabetes Metab 6:601. doi:10.4172/2155-6156.1000601
Copyright: © 2015 Abdirahman YA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Kleinia squarrosa has been used traditionally to manage several diseases including diabetes, however, its efficacy and safety is not well evaluated. The aim of this study was to determine in-vivo hypoglycemic activity and safety of the aqueous stem bark extracts of this plant in male swiss white albino mice. The antidiabetic activity was screened in alloxan induced diabetic mice using oral and intraperitoneal routes. The safety of the extract was studied in mice that were orally and intraperitoneally administered with 1 g/kg body weight daily for 28 days by recording changes in body and organ weights, hematological and biochemical parameters and histology. Mineral composition was estimated using total reflection X-ray fluorescence system (TRXF) and atomic absorption spectrometry (AAS). Phytochemical composition was assessed using standard procedures. The extract showed hypoglycemic activity at dose levels of 50, 100, 200, 300 mg/kg body weight. Administration of 1 g/kg body weight of the extract decreased the body weight gain using both routes, and altered the organ to body weight percentage of the liver and lungs for intraperitoneal route while oral route only altered the liver. Oral administration of the same dose caused a change in levels of RBC, ALP, AST, LDH CK and Creatinine while the same intraperitoneal dose caused a change in RBC, WBC, Hb, PCV, PLT, MCH, MCHC, neutrophils, lymphocytes, eosinophils, monocytes and biochemical parameters: AST, ALT, GGT, LDH, T-BIL, D-BIL, Urea and Creatinine. Moreover, intraperitoneal administration caused significant histological lesions to the kidney, liver and spleen. The extracts contained tannins, phenols, flavonoids, saponins, and alkaloids. Sodium, Chlorine, Potassium, Calcium, Titanium, Vanadium, Chromium, Manganese, Iron, Copper, Zinc, Arsenic, Cadmium, Magnesium, Nickel and Lead were present in the extracts at levels below the recommended daily allowance. The observed hypoglycemic activity and slight toxicity could be associated with the phytochemicals present in this plant extract.