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ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
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Research Article

Isolation and Characterization of Bacteriophage against Methicillin Resistant Staphylococcus aureus

Mariem N Mohammed-Ali* and Nidham M. Jamalludeen

Department of Microbiology, College of Medicine, University of Basrah, Basrah, Iraq

*Corresponding Author:
Mariem N. Mohammed-Ali
Department of Microbiology
College of Medicine, University of Basrah
Basrah, Iraq
Tel: 964 1886 8520
E-mail: [email protected]

Received Date: October 30, 2015; Accepted Date: December 28, 2015; Published Date: December 30, 2015

Citation: Mohammed-Ali MN, Jamalludeen NM (2015) Isolation and Characterization of Bacteriophage against Methicillin Resistant Staphylococcus aureus. J Med Microb Diagn 5:213. doi: 10.4172/2161-0703.1000213

Copyright: © 2015 Mohammed-Ali MN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) is a major human pathogen responsible for several life threatening conditions. MRSA have the ability to acquire resistance to several antimicrobial agents and phage therapy is one potential option to treat this pathogen. The aim of the study was to isolate and characterize bacteriophages effective against a wide range of methicillin-resistant Staphylococcus aureus (MRSA). A mixture of ten MRSA isolates was used for the isolation of phage from wastewater treatment plants. Three phages were selected for further characterization. All three phages belong to the Siphoviridae family and have long non-contractile flexible tails. The three phages showed a wide host range against S. aureus. Phages ɸSA1 and ɸSA2 were resistant to a pH range from 4-10 while ɸSA3 has a pH range from 3-11. DNA from all three phages was resistant to digestion by endonuclease enzymes such as EcoRI and AccI. There was a high degree of mosaicism among the three virulent phages and with their ancestor phages of Siphoviridae due to their non-uniform access to the common genetic pool by horizontal gene transfer and recombination. Since some of the staphylococcal toxins are phage encoded, the presence of genes for such toxins was tested by performing polymerase chain reaction and all three phages lacked genes for any of the staphylococcal toxins, including staphylococcal enterotoxins (sea, seb, sec and see), exfoliating toxins (eta and etb) and the toxic shock syndrome toxin (tst), therefore these bacteriophage are suitable candidates for future use in phage therapy against MRSA.

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