Isolation and Detection of Listeria monocytogenes in Minced Meat, Frozen Chicken and Cheese in Duhok Province, Kurdistan Region by using RT-PCR
- *Corresponding Author:
- Ahmed SS
College of Dairy Science and Technology
Guru Angad Dev Veterinary and Animal Sciences University
Ludhiana - 141004 (Punjab), India
E-mail: [email protected]
Received date February 23, 2016; Accepted date April 26, 2016; Published date May 06, 2016
Citation: Ahmed SS, Tayeb BA, Ameen AM, Merza SM, Sharif YHM (2016) Isolation and Detection of Listeria monocytogenes in Minced Meat, Frozen Chicken and Cheese in Duhok Province, Kurdistan Region by using RT-PCR. J Food Ind Microbiol 2: 109. doi: 10.4172/2572-4134.1000109
Copyright: © 2016 Ahmed SS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Listeria monocytogenes (L. monocytogenes) is the food borne pathogen responsible for listeriosis, which is considered a serious public health risk and are the most important pathogens which can be spread through food products consumption. The disease that can be serious and fatal to human and animals. The objective of present study was carried out to detect, isolate and identify L-monocytogenes from frozen chicken, minced meat and cheese in duhok province. Within a period of six months from march to October 2015, a total of 150 samples were collected including 50 samples of minced meet, 50 samples of frozen chickens and 50 samples of cheese. Biochemical and microbiological test with Real time PCR technique by using specific primer was performed to determine the prevalence of the L-monocytogenes in the samples. Detection of such bacteria in different kinds of food product is crucial to safeguard public health due to it is potential hazard in human and animals. Out of 150 samples 20 samples displayed garish and black colonies with black halos on Oxford and Palcam agar. From the total of 20 suspected Listeria 12 isolate of L. monocytogenes identified by PCR. L. Monocytogenes were detected in 1(2%), 7(14%), and 4(8%) isolates from cheese, minced meat and frozen chicken respectively. The PCR technique has demonstrated to be a rapid and reliable method appropriate for the routine analysis of different types of food.