alexa Isolation of Antioxidant Compounds from Aspergillus Ter
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Isolation of Antioxidant Compounds from Aspergillus Terreus LS01

Rizna Triana Dewi1, Sanro Tachibana2*, Kazutaka Itoh2 and Muhammad Ilyas3

1The United Graduate School of Agriculture Science, Ehime University, 3-5-7 Tarumi Matsuyama, Ehime 790-8566 Japan

2Department of Applied Biosciences, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi Matsuyama, Ehime 790-8566 Japan

3Research Center for Biology - Indonesian Institute of Sciences, Cibinong Science Centre,Jl. Raya Jakarta Bogor KM. 46, Cibinong 16911, Indonesia

*Corresponding Author:
Sanro Tachibana
Departement of Applied Bioscience
Faculty of Agriculture
Ehime University 3-5-7 Tarumi
Matsuyama, Ehime, 790- 8566 Japan
Tel: 81-89-9469864
E-mail: [email protected]

Received Date: December 06, 2011; Accepted Date: January 19, 2012; Published Date: January 25, 2012

Citation: Dewi RT, Tachibana S, Itoh K, Ilyas M (2012) Isolation of Antioxidant Compounds from Aspergillus terreus LS01. J Microbial Biochem Technol 4: 010-014. doi: 10.4172/1948-5948.1000065

Copyright: © 2012 Dewi RT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited



The antioxidative activity of an ethyl acetate extract from Aspergillus terreus LS01was evaluated using various in vitro assays: 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen-peroxide-scavenging free radical scavenging, and β-caroten-linoleat model assay and the antioxidants in the extract were isolated and identified. Silica gel column chromatography was used to separate the extract into five fractions (F1-F5). Fraction 3 possessed significant antioxidative activity with an IC 50 of 19.91μg/ml. Fraction 3 was further separated by column chromatography and yielded the crystalline compounds 1 and 2, which were identified as terreic acid and terremutin, respectively, on the basis of UV-vis spectra, MS and NMR analyses. Terreic acid and terremutin exhibited the highest level of DPPH free radical scavenging activity with IC 50 values of 0.115±4.02 and 0.114±2.19 mM, respectively. These compounds also showed scavenging activity of hydrogen peroxide radicals which terreic acid exhibited higher than terremutin with 74.07±1.48% and 33.74±2.81% respectively. In the β-caroten-linoleat model assay, the inhibition of terreic acid retained 26.01±1.14% and terremutin retained 32.29±2.23%. This is the first report on antioxidative activity of terreic acid and terremutin from A.terreus . The findings indicated that A. terreus could be considered as potential source of natural antioxidant products.

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