alexa Isolation of Bacteriophage against Currently Circulating Strains of Acinetobacter baumannii
ISSN: 2161-0703

Journal of Medical Microbiology & Diagnosis
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Research Article

Isolation of Bacteriophage against Currently Circulating Strains of Acinetobacter baumannii

Jane F Turton1*, Claire Perry1 and Matthew J Hannah2

1Laboratory of HealthCare Associated Infection, Centre for Infections, Health Protection Agency, 61, Colindale Avenue, London NW9 5EQ, United Kingdom

2Virus Reference Department, Health Protection Agency, 61, Colindale Avenue, London NW9 5EQ, United Kingdom

*Corresponding Author:
Turton JF
Laboratory of HealthCare Associated Infection
Centre for Infections,Health Protection Agency, 61
Colindale Avenue,London NW9 5EQ, United Kingdom
Tel: +44 (0)208 327 7224
Fax: +44(0)208 200 7449
E-mail: [email protected]

Received Date: April 03, 2012; Accepted Date: May 08, 2012; Published Date: May 14, 2012

Citation: Turton JF, Perry C, Hannah MJ (2012) Isolation of Bacteriophage against Currently Circulating Strains of Acinetobacter baumannii. J Med Microb Diagn 1:109. doi: 10.4172/2161-0703.1000109

Copyright: © 2012 Turton JF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

We successfully isolated bacteriophage active against clinical strains of Acinetobacter baumannii by enrichichment from activated sewage sludge using representatives of those strains. Purified bacteriophage suspensions obtained were tested on a range of clinical isolates that included representatives of multiple strains of each of the international clonal lineages, as well as minor and sporadic strains. Most representatives of the South East clone, a successful sublineage belonging to international clone II, and a representative of a further strain belonging to international clone II were susceptible to the phage, while further strains belonging to this lineage, as well as those from other lineages were not susceptible. Examination by transmission electron microscopy revealed bacteriophage of the Myoviridae family. Activated sewage sludge is a valuable source of bacteriophages active against some currently circulating strains of A. baumannii.

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