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Isolation of Mature Adipocytes and Stromal Vascular Cells under Adverse Sampling Conditions | OMICS International | Abstract
ISSN: 2167-0943

Journal of Metabolic Syndrome
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Research Article

Isolation of Mature Adipocytes and Stromal Vascular Cells under Adverse Sampling Conditions

Duarte MS1,3#, Wei S2,3#, Paulino PVR1, Du M3, Jiang Z3, Zan L2*, Hausman GJ4 and Dodson MV3*
1Department of Animal Sciences, Universidade Federal de Viçosa, Viçosa, MG 36570–000, Brazil
2College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China
3Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA
4USDA, ARS, Athens, GA 30605, USA
#contributed equally to this effort
Corresponding Authors : Dodson MV
Department of Animal Sciences
Washington State University
Pullman, WA 99164, USA
E–mail: [email protected]
  Zan L
College of Animal Science and Technology
Northwest A&F University, Yangling
Shaanxi Province 712100, China
E–mail: [email protected]
Received July 09, 2012; Accepted August 12, 2012; Published August 16, 2012
Citation: Duarte MS, Wei S, Paulino PVR, Du M, Jiang Z, et al. (2012) Isolation of Mature Adipocytes and Stromal Vascular Cells under Adverse Sampling Conditions. J Metabolic Synd 1:112. doi:10.4172/2167-0943.1000112
Copyright: © 2012 Duarte MS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Methods are described to obtain adipose tissue for cell isolation, under adverse isolation conditions whereby no scientific controls were in place. Such methods could be used by laboratories of institutions where controlled environments (surgery rooms, abattoirs) are not available. While not ideal, we show that a variety of adipocytes and adipocyte-like cells may be isolated from such methods. These types of procedures may facilitate a greater number of persons entering into the research arena with adipocytes, and are easily adaptable to other animal models.

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