Isolation of Trichoderma Spp. from Desert Soil, Biocontrol Potential Evaluation and Liquid Culture Production of Conidia Using Agricultural FertilizersMontoya-Gonzalez AH1,2, Quijano-Vicente G1, Morales-Maza A3, Ortiz-Uribe N2 and Hernandez-Martinez R4*
3Departamento de Cultivos Protegidos, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Campo Experimental Valle de Mexicali, Baja California. México, Carretera a San Felipe Km. 7.5, Colorado Dos, Mexicali, México 21700
- *Corresponding Author:
- Hernandez-Martinez R
Departamento de Microbiología Centro de Investigación
Científica y de Educación Superior de Ensenada Baja California
(CICESE), Carretera Ensenada-Tijuana 3918, Ensenada, México, 22860
Tel: +5216461750500 extn. 27063
E-mail: [email protected]
Received date: January 04, 2016; Accepted date: January 08, 2016; Published date: January 22, 2016
Citation: Montoya-Gonzalez AH, Quijano-Vicente G, Morales-Maza A, Ortiz-Uribe N, Hernandez-Martinez R (2016) Isolation of Trichoderma Spp. from Desert Soil, Biocontrol Potential Evaluation and Liquid Culture Production of Conidia Using Agricultural Fertilizers. J Fertil Pestic 7:163. doi:10.4172/2471-2728.1000163
Copyright: © 2016 Montoya-Gonzalez AH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Three Trichoderma isolates were obtained from sandy soils collected at the “Gran Desierto de Altar” in the northwest of Mexico and characterized by morphologic and molecular analyses as Trichoderma harzianum 8.4, Trichoderma asperellum 12-2 and Trichoderma asperellum BP60. Isolate T. asperellum BP60 inhibited Setophoma terrestris, grew above 50°C, and produced chitinases and siderophores, therefore it was chosen to obtain enough biomass and conidia for field applications. Conidia production was intended in liquid culture fermentation using food grade ingredients and agricultural fertilizers. Assays were done using baffled Erlenmeyer flasks containing 75 mL of culture media, kept under constant agitation at 150 RPM, with initial pH adjusted to 6.5 (NaOH 1N) at 28 ± 2°C and evaluated at 3, 6, 9 and 12 days after inoculation (DAI). Among the carbon sources, sucrose and vinaze; the former induced higher yields of biomass and conidia. Regarding nitrogen sources, the fertilizer (NH4) NO3 induced higher conidia yield. V8 juice (V8) induced the highest effect on production of both biomass and conidia. Therefore, maximum yield was 1.06 × 109 conidia.mL-1, with the formulation with 5 g of KH2PO4 (MKP, Greenhow®), 1.3 g of MgSO4•7H2O (Sul-Mag, Peñoles®), 20 mg of FeCl3•6H2O (Fermont®), 150 ml of V8, 10 ml of vinaze and 2.5 g.L-1 of (NH4)NO3. Results presented here prove the potential for using an alternative, low cost, liquid media to produce conidia of T. asperellum.