alexa Isolation, Purification and Characterization of ÎÂ
ISSN: 2157-7048

Journal of Chemical Engineering & Process Technology
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Research Article

Isolation, Purification and Characterization of β-Glucosidase from Rauvolfia serpentina

Om Prakash Verma1*, Abha Singh1, Nirmala Singh2 and Onkar Chaudhary3
1Jacob School of Biotechnology & Bioengineering, SHIATS, Allahabad
2JDMC, KANPUR
3RTOU, ALLAHABAD
Corresponding Author : Om Prakash Verma
Jacob School of Biotechnology & Bioengineering
Shiats, Allahabad
E-mail: [email protected]
Received September 28, 2011; Accepted November 21, 2011; PublishedNovember 25, 2011
Citation: Verma OP, Singh A, Singh N, Chaudhary O (2011) Isolation, Purification and Characterization of β-Glucosidase from Rauvolfia serpentina. J Chem Eng Process Technol 2:119. doi:10.4172/2157-7048.1000119
Copyright: © 2011 Verma OP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permitsunrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

β-Glucosidase (EC 3.2.1.21) was extracted from different parts of Rauvolfia serpentina and was purified using ammonium sulphate fractional precipitation and Sephadex G-25 chromatography. The molecular weight of different bands in Rauvolfia serpentina was found in the range of 19.4KDa to 92.257KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC while the thermal stability of enzyme β-glucosidase was found to be 30ºC. The activation with FeSO4 is due to Fe++ ions because FeCl3 do not activate the enzyme.

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