Immunotherapy: Open Access
Open Access

Abstract

Joint Action of the Nano-sized System of Small Non-coding RNAs with DDMC Vector and Recombinant IL-7 Reprograms A-549 Lung Adenocarcinoma Cells into CD4⁺ Cells

Oxana V. Klimenko

Small non-coding RNAs, as class of small regulatory molecules, control normal development and differentiation of cells. Micro-RNAs and piwi-interacting-RNAs are members of epigenetic regulators family. In previous studies researches investigated the role of different small non-coding RNAs in pathophysiology of cancer.

Objective: In this study A-549 lung adenocarcinoma cells were firstly epigenetically reprogrammed and transformed into CD4⁺ cells.

Method: I used new non-viral carrier the complex of DDMC vector with antago-miR-155 and piR-30074 for long-lasting transfection of lung cancer cells.

Results: The transformation of A-549 lung cancer cells was proved by morphological and genetic changes (AltAnalyze Platform) in dynamics. I observed CD4⁺ lymphocytes phenotypic marker and OCT4 marker of pluripotent cells by immunofluorescence microscopy in transformed cells.