L-Ascorbic Acid Biosensor Based on Immobilized Enzyme on ZnO Nanorods
Z. H. Ibupoto*, Syed M. Usman Ali, K. Khun and M. Willander
Department of Science and Technology, Campus Norrköping, Linköping University, SE 60174, Norrköping, Sweden
- *Corresponding Author:
- Dr. Z. H. Ibupoto
Department of Science and Technology
Campus Norrköping, Linköping University
Tel: +46 1136 3119
E-mail: [email protected]
Received Date: October 13, 2011; Accepted Date: November, 16 2011; Published Date: November 19, 2011
Citation: Ibupoto ZH, Ali SMU, Khun K, Willander M (2011) L-Ascorbic Acid Biosensor Based on Immobilized Enzyme on ZnO Nanorods. J Biosens Bioelectron 2:110. doi: 10.4172/2155-6210.1000110
Copyright: © 2011 Ibupoto ZH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this present research, we have grown the well aligned, perpendicular to the substrate and highly dense ZnO nanorods on the gold coated substrate by using the hydrothermal growth method. The ZnO nanorods were functionalized by immobilizing the ascorbate oxidase enzyme with cross linking molecule 3-glycidoxypropy1trimethoxysilane (GPTS).The electrochemical measurements were carried out through using potentiometric technique, we observed that the output response exhibited by the biosensor to the wide linear dynamic range concentration of L-ascorbic acid from 1×10 -6 to 5×10 -2 M with good sensitivity of 32 mV/decade. Moreover the biosensor showed excellent fast response time less than 10 seconds, better selectivity, repeatability, reproducibility and no significant interference to the common interfering ions such as potassium K +1 , sodium ions Na +1 , calcium ions Ca +2 , glucose, fructose, and zinc ions Zn +2 etc. except slight interference with copper Cu +2 ions.